LCK 蛋白
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北京 101111
Quick Overview for LCK 蛋白 (ABIN7939235)
抗原
See all LCK 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant LCK protein
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Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) protein (GST tag)
LCK 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Lymphocyte-Specific Protein tyrosine Kinase (LCK) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)LCK 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539), (Isoform 3) protein (His tag)LCK 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant LCK protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
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别名
- LCK
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背景
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Protein Background: LCK (LCK Proto-Oncogene, Src Family Tyrosine Kinase) is a non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. It plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.
Short Description: Recombinant LCK protein was expressed in baculovirus expression system as the full length protein (accession number NP_001036236.1) with a N-terminal FLAG tag. The molecular weight of the protein is 59.3 kDa.
Aliases: LCK Proto-Oncogene, Src Family Tyrosine Kinase
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
抗原
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