Histone 3 Protein (H3K27me3, Nucleosome, pseudo-methylated)
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Quick Overview for Histone 3 Protein (H3K27me3, Nucleosome, pseudo-methylated) (ABIN7939082)
抗原
See all Histone 3 (H3) 蛋白蛋白类型
宿主
资源
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产品特性
- H3K27me3, Nucleosome, pseudo-methylated
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原理
- Recombinant Mononucleosomes H3K27me3 (MLA)
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产品特性
- Recombinant Mononucleosomes H3K27me3 (MLA) consist of a 167 bp of 601 DNA and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3 trimethyl Lys27 (H3K27me3) (MLA) that includes amino acids 1-136 (end) (accession number NP_003520.1) with two substitutions (cysteine to serine at amino acid 96 and cysteine to alanine at amino acid 110), and H4 that includes amino acids 1-103 (end) (accession number NM_003548). All of these histones were expressed in E. coli. The molecular weight of histone octamer is 108 kDa. Nucleosomes comprise the smallest subunit of chromatin, they consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for use in in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.
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Histone H3 (H3) (C110A) protein (His tag)
H3 宿主: 非洲爪蟾 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE PC, S
Histone H3 (H3) (full length), (H3S10ph), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3T3ph), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3K14ac), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3K23ac), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3K18ac), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3K4me2), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3K4me3), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3R8me2a), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
Histone H3 (H3) (full length), (H3K9me1), (truncated) protein (His tag)H3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >98 % pure by SDS-PAGE and confirmed by high-resolution ESI-TOF mass spectrometry PC, S
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应用备注
- Recombinant histones are suitable for use as positive controls in the analysis of histone posttranslational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.
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说明
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Pseudomethylated Lysines have been generated using the partented Methylated Lysin Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine gamma-methylene. The MLA technique provides precise control over the site and degree of methylation.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant Mononucleosomes H3K27me3 (MLA) (20 μg protein + 20 μg DNA) are supplied in 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 2 mM DTT, and 20 % glycerol.
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- Histone 3 (H3) (Histone H3 (H3))
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别名
- Histone H3
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背景
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Protein Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. Recombinant Histone H3K27me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA)technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine Upsilon-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.
Short Description: Recombinant Mononucleosomes H3K27me3 (MLA) consist of a 167 bp of 601 DNA and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3 trimethyl Lys27 (H3K27me3) (MLA) that includes amino acids 1-136 (end) (accession number NP_003520.1) with two substitutions (cysteine to serine at amino acid 96 and cysteine to alanine at amino acid 110), and H4 that includes amino acids 1-103 (end) (accession number NM_003548). All of these histones were expressed in E. coli cells. The molecular weight of histone octamer is 108 kDa.
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分子量
- 108 kDa
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NCBI登录号
- NP_003520
抗原
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