Histone H3.3 Protein (G34R, Nucleosome)
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Quick Overview for Histone H3.3 Protein (G34R, Nucleosome) (ABIN7938946)
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See all Histone H3.3 (H3F3A) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- G34R, Nucleosome
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原理
- Recombinant Polynucleosomes H3.3 (G34R)
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产品特性
- Recombinant Human Polynucleosomes (G34R) protein contains a Gly34Arg point mutation. Nucleosomes comprise the smallest subunit of chromatin, they consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for use in in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.
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H3 Histone, Family 3A (H3F3A) protein (His tag,thrombin cleavage site)
H3F3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % SDS, CyTox, ISt
H3 Histone, Family 3A (H3F3A) (AA 1-136) protein (GST tag)H3F3A 宿主: 人 宿主: 小麦胚 Recombinant ELISA, AP, AA, WB
H3 Histone, Family 3A (H3F3A) (AA 1-136) protein (His tag)H3F3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
H3 Histone, Family 3A (H3F3A) (full length) protein (His tag)H3F3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >92 % pure by SDS-PAGE ScA, AcA, Func
H3 Histone, Family 3A (H3F3A) (full length) protein (Biotin,His tag)H3F3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % pure by SDS-PAGE ScA, AcA, Func
H3 Histone, Family 3A (H3F3A) (AA 2-136), (full length) protein (GST tag)H3F3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % ELISA
H3 Histone, Family 3A (H3F3A) (AA 2-136) protein (His-SUMO Tag)H3F3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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应用备注
- Recombinant Polynucleosomes H3.3 (G34R) are suitable as substrate for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Human Recombinant Polynucleosomes H3.3 (G34R) (20 μg protein+ 24 μg DNA) are supplied in 10 mM Tris pH 8.0, 1 mM EDTA, 2 mM DTT, 20 % glycerol. Please refer to product insert upon arrival for lot-specific concentration.
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))
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别名
- Histone H3.3
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背景
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Protein Background: In vivo, the nucleosome is the basic structural unit of chromatin. It is comprised of about 146 bp of DNA wrapped around a core of eight histones of four different types: H2A, H2B, H3 and H4. Histones are subject to posttranslational modifications, such as methylation, acetylation, phosphorylation and mono-ubiquitination. Histone modifications influence multiple chromatin-templated processes such as gene transcription, DNA repair and recombination. Besides the ""major"" histones, there are some histone variants in specific regions of chromatin or in specific cell types. Histone variants are involved in multiple processes including chromosome segregation, DNA repair, transcriptional regulation and mRNA processing. Histone H3.3 point mutations (K27 and G34) are found in 1/3 of pediatric glioblastomas. Up to 78 % of diffuse intrinsic pontine gliomas (DIPGs) carry K27M and 36 % of non-brainstem gliomas carry either K27M or G34R/V mutations. High- frequency mutation of histone H3 to K36M is observed in chondroblastomas, as is the mutation G34W/L in giant cell tumors of bone, diseases of adolescents and young adults. Histone H3.3 mutations drive pediatric glioblastoma through upregulation of MYCN. Nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.
Short Description: Recombinant Polynucleosomes H3.3 (G34R), Human, consist of 5000 bp of DNA (plasmid pG5E4) and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3.3 that includes amino acids 1-136 (end) (accession number NM_005324) with a point mutation Gly34Arg, and H4 that includes amino acids 1-103 (end) (accession number NM_003548). Plasmid pG5E4 contains 9 of 5S rDNA nucleosome positioning sequences of L. variegatus, 5 of GAL4 binding sites and E4 promoter. Every 5S rDNA can wrap one histone octamer to form a nucleosome. 5 of GAL4 binding sites and E4 promoter can wrap histone octamers to form dinucleosomes. A pG5E4 plasmid can warp histone octamers to form 11 nucleosomes. The recombinant protein is >95 % pure by SDS-PAGE.
Aliases: Histone H3.3, Histone-3, H3, G34R
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