Histone H3.3 Protein (K18I, Nucleosome)
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Quick Overview for Histone H3.3 Protein (K18I, Nucleosome) (ABIN7938939)
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See all Histone H3.3 (H3F3A) 蛋白蛋白类型
宿主
资源
应用范围
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产品特性
- K18I, Nucleosome
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原理
- Recombinant Mononucleosomes H3.3 (K18I)
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H3 Histone, Family 3A (H3F3A) protein (His tag,thrombin cleavage site)
H3F3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % SDS, CyTox, ISt
H3 Histone, Family 3A (H3F3A) (AA 1-136) protein (GST tag)H3F3A 宿主: 人 宿主: 小麦胚 Recombinant ELISA, AP, AA, WB
H3 Histone, Family 3A (H3F3A) (AA 1-136) protein (His tag)H3F3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
H3 Histone, Family 3A (H3F3A) (full length) protein (Biotin,His tag)H3F3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % pure by SDS-PAGE ScA, AcA, Func
H3 Histone, Family 3A (H3F3A) (full length) protein (His tag)H3F3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >92 % pure by SDS-PAGE ScA, AcA, Func
H3 Histone, Family 3A (H3F3A) (AA 2-136), (full length) protein (GST tag)H3F3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % ELISA
H3 Histone, Family 3A (H3F3A) (AA 2-136) protein (His-SUMO Tag)H3F3A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant Mononucleosomes H3.3 (K18I) (20 μg protein + 20 μg DNA) is supplied in 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 2 mM DTT, and 20 % glycerol.
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))
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别名
- Histone H3.3
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背景
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Protein Background: In vivo, histones are wrapped around by DNA in chromatin. Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are signifcantly more active, as well as specifc, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery. Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription.
Short Description: Recombinant Mononucleosomes H3.3 (K18I) consist of a 167 bp of 601 DNA and two molecules each of histones H2A that includes amino acids 1-130 (end) (accession number NM_003512), H2B that includes amino acids 1-126 (end) (accession number NM_003518), H3.3 that includes amino acids 1-136 (end) (accession number NM_005324) with a point mutation Lys18Ile, and H4 that includes amino acids 1-103 (end) (accession number NM_003548). All of these histones were expressed in E. coli cells. The molecular weight of histone octamer is 108 kDa.
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