EIF4A3 蛋白
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Quick Overview for EIF4A3 蛋白 (ABIN7938833)
抗原
See all EIF4A3 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant EIF4A3 protein
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产品特性
- EIF4A3 (Eukaryotic Translation Initiation Factor 4A, Isoform 3), also called as DDX48, NUK34 or RCPS, is a ATP-dependent RNA helicase, which is involved in pre-mRNA splicing as component of the spliceosome. It is a core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The RNA-dependent ATPase and RNA-helicase activities of EIF4A3 are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. This protein is involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. EIF4A3 binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. And it shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 106,314.67 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 152,621.24 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) (AA 1-411) protein (His tag)
custom-made EIF4A3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) (AA 1-411) protein (His tag)custom-made EIF4A3 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) (AA 1-411) protein (GST tag)EIF4A3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) (AA 1-411) protein (His-IF2DI Tag)EIF4A3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) (AA 1-411) protein (His tag)EIF4A3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) protein (Myc-DYKDDDDK Tag)EIF4A3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant EIF4A3 protein is supplied in 25 mM Tris-HCl pH 7.4, 300 mM NaCl,5 % glycerol, 0.1 % Triton X-100.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))
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别名
- EIF4A3
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背景
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Protein Background: EIF4A3 (Eukaryotic Translation Initiation Factor 4A, Isoform 3), also called as DDX48, NUK34 or RCPS, is a ATP-dependent RNA helicase, which is involved in pre-mRNA splicing as component of the spliceosome. It's a core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The RNA-dependent ATPase and RNA-helicase activities of EIF4A3 are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. This protein is involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. EIF4A3 binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. And it shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.
Short Description: Recombinant EIF4A3 protein was expressed in E.coli as the full length protein (accession number NP_055555.1) with an N-terminal 6xHis Tag. The molecular weight of the protein is 50.4 kDa.
Aliases: Eukaryotic Translation Initiation Factor 4A3, DDX48
抗原
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