EIF4A1 蛋白
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北京 101111
Quick Overview for EIF4A1 蛋白 (ABIN7938829)
抗原
See all EIF4A1 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant EIF4A1 protein
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产品特性
- EIF4A1 (Eukaryotic Translation Initiation Factor 4A, Isoform 1), also called as EIF4A or DDX2A, is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition. It is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. This protein is involved in the binding of mRNA to 40S subunit of ribosome, ubiquitous with high expression in skeletal muscle and ovary. The helicase activity of EIF4A1 and function in translation are inhibited by interaction with PDCD4.
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Eukaryotic Translation Initiation Factor 4A1 (EIF4A1) protein (Myc-DYKDDDDK Tag)
EIF4A1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant EIF4A1 protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 20 % glycerol, and 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- EIF4A1 (Eukaryotic Translation Initiation Factor 4A1 (EIF4A1))
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别名
- EIF4A1
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背景
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Protein Background: EIF4A1 (Eukaryotic Translation Initiation Factor 4A, Isoform 1), also called as EIF4A or DDX2A, is a ATP dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition. It is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. This protein is involved in the binding of mRNA to 40S subunit of ribosome,ubiquitous with high expression in skeletal muscle and ovary. The helicase activity of EIF4A1 and function in translation are inhibited by interaction with PDCD4.
Short Description: Recombinant EIF4A1 protein was expressed in E.coli as the full length protein (accession number NP_001407.1) with an N-terminal 6xHis Tag. The molecular weight of the protein is 49.7 kDa.
Aliases: Eukaryotic Translation Initiation Factor 4A1, DDX2A
抗原
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