EEF2 蛋白
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北京 101111
Quick Overview for EEF2 蛋白 (ABIN7938823)
抗原
See all EEF2 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant EEF2 protein
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Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 1-858) protein (GST tag)
EEF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 574-858) protein (His tag)EEF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Eukaryotic Translation Elongation Factor 2 (EEF2) protein (Myc-DYKDDDDK Tag)EEF2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Eukaryotic Translation Elongation Factor 2 (EEF2) (AA 2-858) protein (His tag)EEF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Recombinant EEF2 protein is in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))
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别名
- EEF2
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背景
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Protein Background: EEF2 (Elongation factor 2) promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. Mostly eEF2 is regulated by phosphorylation and dephosphorylation by a specific kinase known as eEF2 kinase, which itself is up regulated by various mechanisms in the eukaryotic cell. The activity of this kinase is dependent on calcium ions and calmodulin. Recently it has been shown that the activity of eEF2 kinase is regulated by MAP kinase signalling and mTOR signalling pathway.
Short Description: Recombinant EEF2 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001952.1) with an N-terminal FLAG tag. The molecular weight of EEF2 is 96.91 kDa.
Aliases: EF2, Elongation Factor 2, EF-2, Epididymis Secretory Binding Protein
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途径
- AMPK Signaling
抗原
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