DAPK3 蛋白
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北京 101111
Quick Overview for DAPK3 蛋白 (ABIN7938758)
抗原
See all DAPK3 蛋白蛋白类型
宿主
资源
应用范围
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原理
- Recombinant DAPK3 / ZIPK protein
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 116,946.14 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 145,985.55 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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Death-Associated Protein Kinase 3 (DAPK3) (AA 1-448) protein (His tag)
custom-made DAPK3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Death-Associated Protein Kinase 3 (DAPK3) (AA 1-454) protein (His tag)custom-made DAPK3 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Death-Associated Protein Kinase 3 (DAPK3) (AA 1-454) protein (GST tag)DAPK3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Death-Associated Protein Kinase 3 (DAPK3) (AA 355-454) protein (GST tag)DAPK3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Death-Associated Protein Kinase 3 (DAPK3) (Active) protein (GST tag)DAPK3 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 85 % as determined by reducing SDS-PAGE. Active
Death-Associated Protein Kinase 3 (DAPK3) protein (Myc-DYKDDDDK Tag)DAPK3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant DAPK3 / ZIPK protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))
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别名
- DAPK3 & ZIPK
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背景
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Protein Background: DAPK3 (Death Associated Protein Kinase 3), a serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. This protein is involved in the regulation of smooth muscle contraction and regulates both type I (caspase-dependent) apoptotic and type II (caspaseindependent) autophagic cell deaths signal, depending on the cellular setting. DAPK3 is involved in regulation of starvation-induced autophagy and regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, DAPK3 regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. DAPK3 phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B.
Short Description: Recombinant DAPK3 / ZIPK protein was expressed in baculovirus expression system as the full length protein (accession number NP_001339.1) with a N-terminal FLAG tag. The molecular weight of the protein is 53.8 kDa.
Aliases: Death Associated Protein Kinase 3, DAP Kinase 3, ZIPK, Ziper interacting protein kinase
抗原
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