CDK4/CyclinD1 (Complex) 蛋白
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北京 101111
Quick Overview for CDK4/CyclinD1 (Complex) 蛋白 (ABIN7938693)
抗原
蛋白类型
宿主
资源
应用范围
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产品特性
- Complex
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原理
- Recombinant CDK4 / Cyclin D1 Complex
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant CDK4 / Cyclin D1 Complex is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- CDK4/CyclinD1
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别名
- CDK4 & Cyclin D1
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背景
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Protein Background: Cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Short Description: Recombinant CDK4 / Cyclin D1 Complex that includes full-length human CDK4 protein (accession number NP_000066.1) with an N-terminal His tag and full-length human Cyclin D1 protein (accession number NP_444284.1) with an C-terminal FLAG tag was expressed in Hi5 cells. The molecular weights of CDK4 and Cyclin D1 are 36.83 kDa and 35.42 kDa, respectively.
抗原
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