CDK2/CyclinE1 (Complex) 蛋白
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北京 101111
Quick Overview for CDK2/CyclinE1 (Complex) 蛋白 (ABIN7938692)
抗原
蛋白类型
宿主
资源
应用范围
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产品特性
- Complex
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原理
- Recombinant CDK2 / Cyclin E1 Complex
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant CDK2 / Cyclin E1 Complex is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- CDK2/CyclinE1
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别名
- CDK2 & Cyclin E1
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背景
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Protein Background: CDK2 is a serine/threonine-protein kinase involved in the control of the cell cycle, essential for meiosis, but dispensable for mitosis. Activity of CDK2 is maximal during S phase and G2, activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. Cyclin E/ CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase.
Short Description: Recombinant CDK2 / Cyclin E1 Complex that includes full length human CDK2 protein (accession number NP_001789.2) with a C-terminal His tag and full-length human Cyclin E1 protein (accession number NP_001229.1) with a C-terminal FLAG tag was expressed in Hi5 cells. The molecular weights of CDK2 and Cyclin E1 are 35.57 kDa and 48.77 kDa, respectively.
抗原
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