AGO2 Protein (DYKDDDDK Tag)
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Quick Overview for AGO2 Protein (DYKDDDDK Tag) (ABIN7938606)
抗原
See all AGO2 蛋白蛋白类型
宿主
资源
应用范围
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标记
- This AGO2 protein is labelled with DYKDDDDK Tag.
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原理
- Recombinant AGO2 protein
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产品特性
- Recombinant AGO2 (accession number NP_036286.2) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 104 kDa. The recombinant protein is >90 % pure by SDS-PAGE.
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Argonaute 2 (AGO2) (AA 1-860) protein (His tag)
custom-made AGO2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Argonaute 2 (AGO2) (AA 381-480) protein (GST tag)AGO2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Argonaute 2 (AGO2) protein (His tag)AGO2 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 88 % as determined by SDS-PAGE
Argonaute 2 (AGO2) protein (DYKDDDDK Tag)AGO2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant AcA, Func, ScA
Argonaute 2 (AGO2) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)AGO2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Recombinant AGO2 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
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注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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储存条件
- -80 °C
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储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- AGO2 (Argonaute 2 (AGO2))
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别名
- AGO2
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背景
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Protein Background: AGO2 (Argonaute RISC Catalytic Component 2, also known as Q10, EIF2C2) is a member of the Argonaute family of proteins which play a role in RNA interference. This process is important both in gene regulation by microRNAs and in defense against viral infections. The RNA-induced silencing complex, or RISC, is a multiprotein complex that includes argonaute proteins and one strand of a small interfering RNA (siRNA) or microRNA (miRNA). RISC uses the siRNA or miRNA as a template for recognizing complementary mRNA. When it recognizes a complementary strand, it activates AGO2 to cleave the mRNA. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. In some cases, RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'UTR). In addition, AGO2 can also up-regulate the posttranscriptional upregulation of specific mRNAs under certain growth conditions.
Short Description: Recombinant AGO2 (accession number NP_036286.2) was expressed in Sf9 cells and contains an N-terminal Flag tag with a molecular weight of 105 kDa. The recombinant protein is > 85 % pure by SDS-PAGE.
Aliases: Q10, EIF2C2
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分子量
- 104 kDa
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NCBI登录号
- NP_036286
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途径
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization
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