Osteopontin Protein (AA 17-300)
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北京 101111
Quick Overview for Osteopontin Protein (AA 17-300) (ABIN7956564)
抗原
See all Osteopontin (SPP1) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 17-300
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原理
- Recombinant Human Osteopontin/OPN
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序列
- Ile17-Asn300, with a C-terminal 6-His tag, Accession # NP_000573.1
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过滤
- 0.2 μm filtered
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内毒素水平
- Less than 0.1 EU/ug of rHuOsteopontin/OPN as determined by LAL method.
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Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x105 cells/well are added to a rHuOsteopontin/OPN coated plate, cell adhesion is enhanced in a dosedependent manner after 1 hour incubation at 37˚,C. The ED50 for this effect is typically 0.1-0.6 μg/mL. rHuThrombin proteolytic treatment of this rHuOsteopontin can increase HEK293 cell adhesion by about 5-fold.
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Secreted phosphoprotein 1 (SPP1) (AA 17-300) (Active) protein (His tag)
SPP1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Secreted phosphoprotein 1 (SPP1) (AA 1-300) protein (GST tag)SPP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Secreted phosphoprotein 1 (SPP1) proteinSPP1 宿主: 人 Native > 90 % (SDS-PAGE)
Secreted phosphoprotein 1 (SPP1) (AA 28-61) protein (His-IF2DI Tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Secreted phosphoprotein 1 (SPP1) (AA 650-920) protein (His-IF2DI Tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Secreted phosphoprotein 1 (SPP1) (AA 17-287) (Active) protein (His tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
Secreted phosphoprotein 1 (SPP1) (AA 17-317) (Active) protein (His tag)SPP1 宿主: 大鼠 宿主: HEK-293 Cells > 95 % AcA, CC Active
Secreted phosphoprotein 1 (SPP1) (AA 17-300), (partial) protein (His tag)SPP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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说明
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Apparent molecular mass of 60-65 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 290 amino acids including a C-terminal 6x polyhistidine tag.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Lyophilized from 0.2 um filtered concentrated solution in PBS, pH 7.2.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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有效期
- 12 months
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- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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别名
- Osteopontin & OPN
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分子量
- 65 kDa
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途径
- Regulation of Cell Size
抗原
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