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LIF 蛋白

This Recombinant LIF protein is expressed in 大肠杆菌(E. Coli).
产品编号 ABIN7937415
发货至: 中国
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Quick Overview for LIF 蛋白 (ABIN7937415)

抗原

See all LIF 蛋白
LIF (Leukemia Inhibitory Factor (LIF))

蛋白类型

Recombinant

生物活性

Active

宿主

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资源

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大肠杆菌(E. Coli)

纯度

> 98 % by SDS-PAGE and HPLC analyses.
  • 原理

    Recombinant Murine Leukemia Inhibitory Factor

    序列

    SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF

    过滤

    0.2 μm filtered

    内毒素水平

    Less than 1 EU/ug of rMuLIF as determined by LAL method.

    Biological Activity Comment

    Fully biologically active when compared to standard. The specific activity is determined by inducing differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of rMuLIF in this assay is 0.01 ng/ml. The specific activity of > 1.0 x108 IU/mg, where 50 units is defined as the amount of rMuLIF required to induce differentiation in 50 % of the M1 colonies in 1 ml agar cultures.
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  • 说明

    Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.

    限制

    仅限研究用
  • 状态

    Lyophilized

    缓冲液

    Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, with 0.02 % TWEEN 20.

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    有效期

    12 months
  • 抗原

    LIF (Leukemia Inhibitory Factor (LIF))

    别名

    Leukemia Inhibitory Factor

    分子量

    19.9 kDa

    基因ID

    16878

    UniProt

    P09056

    途径

    JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
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