CRISPR-Cas9 protein (His tag)
This Recombinant CRISPR-Cas9 protein is expressed in 大肠杆菌（E. Coli）.
产品编号 ABIN7884339
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中国
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Quick Overview for CRISPR-Cas9 protein (His tag) (ABIN7884339)
抗原
CRISPR-Cas9
蛋白类型
Recombinant
宿主
Streptococcus pyogenes
资源
大肠杆菌（E. Coli）
应用范围
Western Blotting (WB)
纯度
>98 %
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标记
- His tag
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原理
- wtCas9
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序列
- MDKKYSIGLD IGTNSVGWAV ITDEYKVPSK KFKVLGNTDR HSIKKNLIGA LLFDSGETAE ATRLKRTARR RYTRRKNRIC YLQEIFSNEM AKVD DSFFHRLEES FLVEEDKKHE RHPIFGNIVD EVAYHEKYPT IYHLRKKLVD STDKADLRLI YLALAHMIKF RGHFLIEGDL NPDNSDVDKL FIQLVQ TYNQLFEENP INASGVDAKA ILSARLSKSR RLENLIAQLP GEKKNGLFGN LIALSLGLTP NFKSNFDLAE DAKLQLSKDT YDDDLDNLLA QIGD QYADLFLAAK NLSDAILLSD ILRVNTEITK APLSASMIKR YDEHHQDLTL LKALVRQQLP EKYKEIFFDQ SKNGYAGYID GGASQEEFYK FIKPILE KMDGTEELLV KLNREDLLRK QRTFDNGSIP HQIHLGELHA ILRRQEDFYP FLKDNREKIE KILTFRIPYY VGPLARGNSR FAWMTRKSEE TITPW NFEEVVDKGA SAQSFIERMT NFDKNLPNEK VLPKHSLLYE YFTVYNELTK VKYVTEGMRK PAFLSGEQKK AIVDLLFKTN RKVTVKQLKE DYFK KIECFDSVEI SGVEDRFNAS LGTYHDLLKI IKDKDFLDNE ENEDILEDIV LTLTLFEDRE MIEERLKTYA HLFDDKVMKQ LKRRRYTGWG RLSRKLI NGIRDKQSGK TILDFLKSDG FANRNFMQLI HDDSLTFKED IQKAQVSGQG DSLHEHIANL AGSPAIKKGI LQTVKVVDEL VKVMGRHKPE NIV IEMARENQTT QKGQKNSRER MKRIEEGIKE LGSQILKEHP VENTQLQNEK LYLYYLQNGR DMYVDQELDI NRLSDYDVDH IVPQSFLKDD SI DNKVLTRSDK NRGKSDNVPS EEVVKKMKNY WRQLLNAKLI TQRKFDNLTK AERGGLSELD KAGFIKRQLV ETRQITKHVA QILDSRMNTK Y DENDKLIREV KVITLKSKLV SDFRKDFQFY KVREINNYHH AHDAYLNAVV GTALIKKYPK LESEFVYGDY KVYDVRKMIA KSEQEIGKAT AKYFF YSNIMNFFKT EITLANGEIR KRPLIETNGE TGEIVWDKGR DFATVRKVLS MPQVNIVKKT EVQTGGFSKE SILPKRNSDK LIARKKDWDP KKYG GFDSPTVAYS VLVVAKVEKG KSKKLKSVKE LLGITIMERS SFEKNPIDFL EAKGYKEVKK DLIIKLPKYS LFELENGRKR MLASAGELQK GNELALP SKYVNFLYLA SHYEKLKGSP EDNEQKQLFV EQHKHYLDEI IEQISEFSKR VILADANLDK VLSAYNKHRD KPIREQAENI IHLFTLTNLG APAAFK YFDTTIDRKR YTSTKEVLDA TLIHQSITGL YETRIDLSQL GGD with polyhistid ine tag at the C-terminus
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内毒素水平
- <0.1 EU per 1 μg protein
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- dissolve in sterile H2O
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缓冲液
- PBS, pH 7.4
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储存条件
- -20 °C
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储存方法
- -20°C
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- CRISPR-Cas9
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别名
- Cas9
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背景
- The wtCas9 is a recombinant Streptococcus pyogenes Cas9 protein (wild type form) purified from E. coli. Cas9 protein can form a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA). The RNP complex recognizes the target site by matching gRNA with the genomic DNA sequence and leads to DNA breaks within 3 bases from the NGG PAM (Protospacer Adjacent Motif). With the CRISPR/Cas9 system, researchers can valid gene editing assay in cells or other kinds of animals.
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分子量
- 158kDa
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UniProt
- Q99ZW2
抗原
-
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