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Osteopontin Protein (AA 22-323)

This Recombinant Osteopontin protein is expressed in 大肠杆菌(E. Coli).
产品编号 ABIN7866059
发货至: 中国
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Quick Overview for Osteopontin Protein (AA 22-323) (ABIN7866059)

抗原

See all Osteopontin (SPP1) 蛋白
Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

蛋白类型

Recombinant

生物活性

Active

宿主

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大肠杆菌(E. Coli)

纯度

>95 % , as determined by SDS-PAGE and HPLC
  • 产品特性

    AA 22-323

    原理

    Human SPP1 Protein Recombinant

    序列

    VHHPPSYVAH LASDFGVRVF QQVAQASKDR NVVFSPYGVA SVLAMLQLTT GGETQQQIQA AMGFKIDDKG MAPALRHLYK ELMGPWNKDE ISTTDAIFVQ RDLKLVQGFM PHFFRLFRST VKQVDFSEVE RARFIINDWV KTHTKGMISN LLGKGAVDQL TRLVLVNALY FNGQWKTPFP DSSTHRRLFH KSDGSTVSVP MMAQTNKFNY TEFTTPDGHY YDILELPYHG DTLSMFIAAP YEKEVPLSAL TNILSAQLIS HWKGNMTRLP RLLVLPKFSL ETEVDLRKPL ENLGMTDMFR QFQADFTSLS DQEPLHVAQA LQKVKIEVNE SGTVASSSTA VIVSARMAPE EIIMDRPFLF VVRHNPTGTV LFMGQVMEP

    产品特性

    Protein Size: Recombinant SPP1 migrates as an approximately 42 kDa protein under reducing conditions in SDS-PAGE.

    Sequence Position: Optimized DNA sequence encoding Human SPP1 mature chain was expressed in E. Coli. Recombinant Human C-type lectin domain family 11 member A encodes amino acid residues 22-323.

    内毒素水平

    Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

    Biological Activity Comment

    Determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37°C.
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  • 限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

    缓冲液

    SPP1 was Lyophilized from PBS, pH 7.2.

    储存条件

    4 °C,-20 °C

    储存方法

    The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

    有效期

    24 months
  • 抗原

    Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

    别名

    SPP1

    分子量

    42 kDa

    途径

    Regulation of Cell Size
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