BMP6 蛋白
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北京 101111
Quick Overview for BMP6 蛋白 (ABIN7865993)
抗原
See all BMP6 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- Human BMP6 Protein Recombinant
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产品特性
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Protein Size: Native humanBMP-6 isgenerated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately17 kDa. Recombinant BMP-6 is a disulfide-linked homodimeric protein consisting of two 140 amino acid residue subunits,and migrates as an approximately34 kDa protein under non-reducing conditions and as a 17 kDa protein under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human Bone Morphogenetic protein-6 mature chain was expressed in Escherichia Coli.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- The ED(50) was determined by its ability to induce alkaline phosphatase production by mouse ATDC5 chondrogenic and was found to be in the range of0.1-0.5 ug/ml.
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Bone Morphogenetic Protein 6 (BMP6) (AA 21-513) protein (His tag)
BMP6 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Bone Morphogenetic Protein 6 (BMP6) (AA 21-510) protein (His tag)BMP6 宿主: 小鼠 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Bone Morphogenetic Protein 6 (BMP6) (AA 375-513) protein (His-IF2DI Tag)BMP6 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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缓冲液
- Recombinant Bone morphogenetic Protein-6was lyophilized from a 0.2 μm filtered solution in 2.5 % glycine, 0.5 % sucrose, 0.01 % Tween80, 5 mM Glutamic acid, pH 4.5.
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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有效期
- 24 months
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- BMP6 (Bone Morphogenetic Protein 6 (BMP6))
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别名
- BMP6
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分子量
- 34 kDa
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途径
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process
抗原
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