IgE 蛋白
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北京 101111
Quick Overview for IgE 蛋白 (ABIN7855496)
抗原
See all IgE 蛋白蛋白类型
宿主
资源
应用范围
纯度
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特异性
- 4-Hydroxy-3-nitrophenylacetyl (NP)
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灵敏度
- 100%, compared to calibrator (Internal Standard IgE (JW8)), tested by SPR (Biacore™) binding to immobilized recombinant human high affinity IgE receptor
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产品特性
- Clone: NBS-U3361851G0, Isotype: Human FcEpsilon with murine kappa
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纯化方法
- Affinity - purified from rec. cell culture supernatant by Immunoaffinity Chromatography (Select IgE Affinity Matrix+HiLoad 26/600 Superdex 200pg)
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IgE (AA 209-428) protein (His-Avi Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 209-429) (Active) protein (His-Avi Tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 91-421) (Active) protein (His tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 198-421) (Active) protein (His-Avi Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE protein (Biotin)宿主: 人 宿主: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, FACS
IgE (AA 1-429) protein (His tag)宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-432) protein (His tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
IgE (AA 1-428) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS); pH = 7.2; 0.1% NaN3 (sodium azide)
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Aliquot to Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C/-80 °C
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- IgE
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物质类
- Antibody
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背景
- Recombinant chimeric IgE consisting of a human Fc heavy chain linked to the antigen binding variable region of a mouse hybridoma by in vitro DNA recombination. The antibody is specific for the hapten 4-Hydroxy-3-nitrophenylacetyl (NP). IgE (JW8) recognizes the human high affinity IgE receptor (FcRI) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with NP-BSA conjugate as specific cross-linking agent.
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基因ID
- 3497
抗原
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