TSPAN33 Protein (DYKDDDDK Tag)
This MNP Membrane Nanoparticle TSPAN33 protein is expressed in HEK-293 Cells.
产品编号 ABIN7853967
发货至:
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Quick Overview for TSPAN33 Protein (DYKDDDDK Tag) (ABIN7853967)
抗原
See all TSPAN33 蛋白
TSPAN33
(Tetraspanin 33 (TSPAN33))
蛋白类型
MNP Membrane Nanoparticle
宿主
人
资源
HEK-293 Cells
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标记
- This TSPAN33 protein is labelled with DYKDDDDK Tag.
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原理
- Human TSPAN33 full length protein-MNP
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产品特性
- Plasma membrane-coated nanoparticles (MNPs) have been used in various applications, including delivery of therapeutic agents and induction of immune responses et al. Unlike the conventional strategies, MNPs directly leverage intact and natural functions of cell membranes, and show high biocompatibility, specificity, and low side effects. Our optimized MNPs platform for the full-length membrane protein production uses membrane coating technology and a HEK293 based expression platform. The high-purity plasma membrane-coated nanoparticles were produced by extrusion after membrane extraction from the host HEK293 cells containing the overexpressed target proteins.
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Tetraspanin 33 (TSPAN33) protein (DYKDDDDK Tag)
TSPAN33 宿主: 人 宿主: HEK-293 Cells Synthetic Nanodisc ELISA, cryo-EM, Imm, PhD, SPR
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说明
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Advantages of MNP:
- High display density of target membrane proteins
- Native structure and orientation of transmembrane protein
- soluble in aqueous solutions for routine biochemical analysis
- Detergent-free purification process
- Strong immunogenicity
- Works for MPs that can´t be produced via VLPs and EXos
- Lack of accurate quantification of the target membrane proteins.
- Need to develop special SPR assayx.
- Cell membranes contain housekeeping proteins that can result in immune response dilution.
- Some membrane proteins can´t be enriched on membrane.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Lyophilized from PBS. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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有效期
- 12 months
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- TSPAN33 (Tetraspanin 33 (TSPAN33))
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别名
- TSPAN33
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背景
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PEN, PEN., TSPAN-33
Plays an important role in normal erythropoiesis (By similarity). It has a role in the differentiation of erythroid progenitors (By similarity). Regulates maturation and trafficking of the transmembrane metalloprotease ADAM10 (PubMed:26686862). Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity (PubMed:26686862). -
分子量
- The human full length TSPAN33 protein has a MW of 31.5 kDa
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UniProt
- Q86UF1
抗原
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