Leptin Protein (LEP)
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北京 101111
Quick Overview for Leptin Protein (LEP) (ABIN7849713)
抗原
See all Leptin (LEP) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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原理
- Recombinant Human Leptin Protein
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过滤
- 0.2 μm filtered
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内毒素水平
- <1 EU/μg by LAL test
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Leptin (LEP) (AA 22-167) (Active) protein (hFc Tag)
LEP 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Leptin (LEP) (AA 22-167) protein (GST tag)LEP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Leptin (LEP) protein (His tag)LEP 宿主: Goldfish 宿主: 酵母菌 Recombinant > 95 % as determined by reducing SDS-PAGE.
Leptin (LEP) proteinLEP 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 22-167) protein (Fc Tag)LEP 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Leptin (LEP) (AA 22-167) protein (His-IF2DI Tag)LEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP 宿主: Pig 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 428-635) protein (His-IF2DI Tag)LEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) proteinLEP 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water(It is not recommended to reconstitute to a concentration<100 μg/mL.)
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缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl,50 mM NaCl,10 % Trehalose,0.02 %Tween80, pH 8.5.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Ship at 4°C.Upon receipt, aliquot and store at -20°C or -80°C for long term. Avoid repeated freeze and thaw cycles.
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- Leptin (LEP)
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别名
- Leptin
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背景
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Synonyms: LEP, LEPD, OB, OBS, leptin
Description: Recombinant human leptin is produced by the E.coli system, with the target gene encoding Val22-Cys167 expressed.
Background: Leptin, also known as obese protein,is a protein hormone predominantly made by adipocytes (cells of adipose tissue). Its primary role is likely to regulate long-term energy balance.As one of the major signals of energy status, leptin levels influence appetite, satiety, and motivated behaviors oriented toward the maintenance of energy reserves (e.g., feeding, foraging behaviors).The amount of circulating leptin correlates with the amount of energy reserves, mainly triglycerides stored in adipose tissue. High leptin levels are interpreted by the brain that energy reserves are high, whereas low leptin levels indicate that energy reserves are low, in the process adapting the organism to starvation through a variety of metabolic, endocrine, neurobiochemical, and behavioral changes.
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UniProt
- P41159
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途径
- JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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