IgE Protein (Biotin)
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Quick Overview for IgE Protein (Biotin) (ABIN7827980)
抗原
See all IgE 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This IgE protein is labelled with Biotin.
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特异性
- Binds with Fc effector domain to human and non human primate IgE receptors, no cross-reactivity with rodent or canine IgE receptors, specific antigen unknown
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灵敏度
- Reactivity > 99 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone SUS-11)
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产品特性
- IGHE, chromosome 14q32.33 Source: Human B cell hybridoma from healthy donors (CD40 cell culture system)
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纯化方法
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - mouse anti-human IgE mAb (BSW17)
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IgE (AA 209-428) protein (His-Avi Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 209-429) (Active) protein (His-Avi Tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 91-421) (Active) protein (His tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE (AA 198-421) (Active) protein (His-Avi Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
IgE protein (Biotin)宿主: 人 宿主: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, FACS
IgE (AA 1-429) protein (His tag)宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-428) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
IgE (AA 1-432) protein (His tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
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应用备注
- For functional cellular assays (mast cell / basophil activation)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH=7.2, azide free
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储存液
- Azide free
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注意事项
- Aliquot to Avoid repeated freezing and thawing.
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储存条件
- 4 °C
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- IgE
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背景
- Recombinant human IgE to be used as reference for IgE concentration measurements in biological samples (ELISA, FACS). IgE (SUS-11) recognizes the human high affinity IgE receptor (FceRIa), the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells
抗原
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