Ubiquitin Protein (Ub) (His tag)
This Recombinant Ubiquitin protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for Ubiquitin Protein (Ub) (His tag) (ABIN7669930)
抗原
See all Ubiquitin (Ub) 蛋白
Ubiquitin (Ub)
蛋白类型
Recombinant
生物活性
Inactive
宿主
人
资源
大肠杆菌（E. Coli）
纯度
> 95 %
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标记
- This Ubiquitin protein is labelled with His tag.
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原理
- Recombinant Human Ub protein (His tag)
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序列
- Lys 103-Cys 229
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纯化方法
- > 95 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 10 EU/mg of the protein as determined by the LAL method.
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Ubiquitin (Ub) protein
Ub 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Ubiquitin (Ub)
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别名
- Ub
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背景
- Polyubiquitin-B(UBB) is one of Ubiquitins. Ubiquitin is one of the most conserved proteins known in eukaryotic organisms. Ubiquitin is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.,Polyubiquitin-B,UBB,Ubiquitin,Ubiquitin B
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分子量
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calculated_mw: 13.9 kDa
observed_mw: 15 kDa
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UniProt
- P0CG47
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途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
抗原
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