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Ubiquitin Protein (Ub) (His tag)

This Recombinant Ubiquitin protein is expressed in 大肠杆菌(E. Coli).
产品编号 ABIN7669930
发货至: 中国
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Quick Overview for Ubiquitin Protein (Ub) (His tag) (ABIN7669930)

抗原

See all Ubiquitin (Ub) 蛋白
Ubiquitin (Ub)

蛋白类型

Recombinant

生物活性

Inactive

宿主

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资源

  • 7
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大肠杆菌(E. Coli)

纯度

> 95 %
  • 标记

    This Ubiquitin protein is labelled with His tag.

    原理

    Recombinant Human Ub protein (His tag)

    序列

    Lys 103-Cys 229

    纯化方法

    > 95 % as determined by reducing SDS-PAGE.

    过滤

    0.2 μm filtered

    内毒素水平

    < 10 EU/mg of the protein as determined by the LAL method.
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  • 限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

    缓冲液

    Lyophilized from sterile PBS, pH 7.4.
    Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

    有效期

    12 months
  • 抗原

    Ubiquitin (Ub)

    别名

    Ub

    背景

    Polyubiquitin-B(UBB) is one of Ubiquitins. Ubiquitin is one of the most conserved proteins known in eukaryotic organisms. Ubiquitin is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.,Polyubiquitin-B,UBB,Ubiquitin,Ubiquitin B

    分子量

    calculated_mw: 13.9 kDa

    observed_mw: 15 kDa

    UniProt

    P0CG47

    途径

    Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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