SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 319-541, E484K, K417N, N501Y, Receptor Binding Domain) (His tag)
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Quick Overview for SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 319-541, E484K, K417N, N501Y, Receptor Binding Domain) (His tag) (ABIN7669657)
抗原
See all SARS-CoV-2 Spike (SARS-CoV-2 S) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 319-541, E484K, K417N, N501Y, Receptor Binding Domain
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标记
- This SARS-CoV-2 Spike protein is labelled with His tag.
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原理
- Recombinant SARS-CoV-2 Spike RBD(K417N, E484K, N501Y)(His Tag)
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序列
- Arg319-Phe541
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纯化方法
- > 90 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Immobilized human ACE2 protein (Fc tag)(Cat: PKSH031870) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(K417N, E484K, N501Y)-His (PKSV030368), the EC50 of PKSV030368 is 5-40 ng/mL.
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SARS-CoV-2 Spike (SARS-CoV-2 S) (Super Stable Trimer) protein (His tag)
SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 16-1188), (Trimer) (Active) protein (His-Avi Tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-532), (RBD) (Active) protein (His-Avi Tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-541), (B.1.1.529 - Omicron), (RBD) (Active) protein (His tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-541), (E484K), (K417N), (N501Y), (RBD) (Active) protein (His tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-541), (B.1.1.7 - alpha), (RBD) protein (Fc Tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. IA, AM, ADA, ScA
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-541), (B.1.617.2 - delta), (RBD) protein (Fc Tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. IA, AM, ADA, ScA
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-541), (RBD) protein (His tag,mFc Tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. IA, AM, ADA, ScA
SARS-CoV-2 Spike (SARS-CoV-2 S) (Super Stable Trimer) protein (His tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (SARS-CoV-2 S) (AA 319-541), (B.1.640.2), (RBD) (Active) protein (His tag)SARS-CoV-2 S 宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- SARS-CoV-2 Spike (SARS-CoV-2 S)
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别名
- SARS-CoV-2 Spike
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物质类
- Viral Protein
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背景
- The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, dipeptidyl peptidase-4, APN, aminopeptidase N, CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1, Sia, sialic acid, O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion, Defines the range of the hosts and specificity of the virus, Main component to bind with the neutralizing antibody, Key target for vaccine design, Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.,2019-nCoV S protein RBD,S protein RBD,SARS-CoV-2 Spike RBD
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分子量
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calculated_mw: 26.6 kDa
observed_mw: 35 kDa
抗原
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