Prosaposin Protein (PSAP) (His tag)
Quick Overview for Prosaposin Protein (PSAP) (His tag) (ABIN7669489)
抗原
See all Prosaposin (PSAP) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This Prosaposin protein is labelled with His tag.
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原理
- Recombinant Human PSAP protein (His tag)
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序列
- Gly 17-Asn 524
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纯化方法
- > 95 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 10 EU/mg of the protein as determined by the LAL method.
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Prosaposin (PSAP) (AA 18-524) protein (GST tag)
PSAP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Prosaposin (PSAP) (AA 60-197) protein (His-IF2DI Tag)PSAP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Prosaposin (PSAP) (AA 1-524) protein (GST tag)PSAP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Prosaposin (PSAP) protein (His tag)PSAP 宿主: 人 宿主: HEK-293 Cells Recombinant > (79.8+19.7) % as determined by reducing SDS-PAGE..
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Prosaposin (PSAP)
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别名
- PSAP
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背景
- This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.,GLBA,PSAP,SAP1p,proactivator polypeptide,prosaposin
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分子量
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calculated_mw: 55.8 kDa
observed_mw: 45 kDa
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UniProt
- P07602
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途径
- Positive Regulation of Endopeptidase Activity
抗原
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