Phenylalanine Hydroxylase Protein (His tag)
This Recombinant Phenylalanine Hydroxylase protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for Phenylalanine Hydroxylase Protein (His tag) (ABIN7669419)
抗原
See all Phenylalanine Hydroxylase 蛋白
Phenylalanine Hydroxylase
蛋白类型
Recombinant
生物活性
Inactive
宿主
人
资源
大肠杆菌（E. Coli）
纯度
> 95 %
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标记
- This Phenylalanine Hydroxylase protein is labelled with His tag.
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原理
- Recombinant Human PAH protein (His tag)
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序列
- Ser 2-Lys 452
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纯化方法
- > 95 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 10 EU/mg of the protein as determined by the LAL method.
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Phenylalanine Hydroxylase (AA 1-452) protein (GST tag)
宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Phenylalanine Hydroxylase protein (Myc-DYKDDDDK Tag)宿主: 化学剂 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Phenylalanine Hydroxylase
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别名
- PAH
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背景
- PAH (phenylalanine hydroxylase), also known as PH, belongs to the biopterin-dependent aromatic amino acid hydroxylase family. It contains 1 ACT domain, N-terminal region of PAH is thought to contain allosteric binding sites for phenylalanine and to constitute an "inhibitory" domain that regulates the activity of a catalytic domain in the C-terminal portion of the molecule. In humans, PAH is expressed both in the liver and the kidney, and there is some indication that it may be differentially regulated in these tissues. PAH catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. It is one of three members of the pterin-dependent amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin and a non-heme iron for catalysis. Defects in PAH are the cause of phenylketonuria (PKU). PKU is an autosomal recessive inborn error of phenylalanine metabolism, due to severe phenylalanine hydroxylase deficiency. It is characterized by blood concentrations of phenylalanine persistently above 1200 mumol.,PH,PKU,PKU1
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分子量
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calculated_mw: 49.5 kDa
observed_mw: 50 kDa
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UniProt
- P00439
抗原
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