Occludin Protein (OCLN) (His tag)
Quick Overview for Occludin Protein (OCLN) (His tag) (ABIN7669390)
抗原
See all Occludin (OCLN) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This Occludin protein is labelled with His tag.
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原理
- Recombinant Human OCLN Protein(His Tag)
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序列
- Lys266~Thr522
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纯化方法
- SDS-PAGE analysis of Human OCLN proteins, 2 μg/lane of Recombinant Human OCLN proteins was resolved with an SDS-PAGE under reducing conditions, showing bands at 28 KD
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过滤
- 0.2 μm filtered
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内毒素水平
- < 10 EU/mg of the protein as determined by the LAL method.
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Occludin (OCLN) (AA 1-521) protein (His tag)
custom-made OCLN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Occludin (OCLN) (AA 1-522) protein (His tag)custom-made OCLN 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Occludin (OCLN) (AA 1-522) protein (GST tag)OCLN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Occludin (OCLN) (AA 270-441) protein (His-IF2DI Tag)OCLN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Occludin (OCLN) (AA 423-522) protein (GST tag)OCLN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Occludin (OCLN)
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别名
- OCLN
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背景
- This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with an simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.,BLCPMG,FLJ08163,FLJ18079,FLJ77961,FLJ94056,MGC34277,Occludin,Ocln,OCLN,Phosphatase 1 regulatory subunit 115,PPP1R115,PTORCH1,Tight junction protein occludin
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分子量
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calculated_mw: 28 kDa
observed_mw: 42 kDa
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UniProt
- Q16625
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途径
- Cell-Cell Junction Organization, Hepatitis C
抗原
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