Neurturin Protein (NRTN) (His tag)
This Recombinant Neurturin protein is expressed in 大肠杆菌（E. Coli）.
Quick Overview for Neurturin Protein (NRTN) (His tag) (ABIN7669369)
抗原
See all Neurturin (NRTN) 蛋白
Neurturin (NRTN)
蛋白类型
Recombinant
生物活性
Active
宿主
小鼠
资源
大肠杆菌（E. Coli）
应用范围
Cell Culture (CC)
纯度
> 98 %
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标记
- This Neurturin protein is labelled with His tag.
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原理
- Recombinant Mouse Neurturin protein(His Tag)
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序列
- Pro 96-Val 195
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过滤
- 0.2 μm filtered
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内毒素水平
- < 0.1 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measure by its ability to induce proliferation in SH-SY5Y cells.The ED50 for this effect is <50 ng/mL.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Neurturin (NRTN)
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别名
- Neurturin
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背景
- Neurturin is a member of the GDNF family of ligands, which include glial cell-derived neurotrophic factor (GDNF), Neurturin, Persephin, and Artemin. Neurturin is expressed in both neuronal and nonneuronal tissues. Similarly to other TGFβ family proteins, Neurturin is synthesized as a precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxyterminal domain. The carboxy terminal domain of Neurturin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active human Neurturin is a disulfide-linked homodimer of the carboxy-terminal 102 amino acid residues. Unlike other members of TGF-β family, bioactivities of all GDNF family ligands are mediated through a unique multicomponent receptor complex composed of high affinity ligand binding component (GFRα-1-GFRα-4) and a common signaling component (cRET receptor tyrosine kinase). Each member of the GDNF family ligands has its preferred binding protein. Neurturin preferentially binds to GFRα-2 but can also bind GFRα-1 at higher concentrations. It may play a role in regulating the development and maintenance of the central and peripheral nervous systems and as well as non neuronal systems.,JE,MCP-1,Monocyte Chemotactic Protein-1
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分子量
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calculated_mw: 12.3 kDa
observed_mw: 11-17 kDa
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UniProt
- P97463
抗原
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