NOV Protein (His tag)
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北京 101111
Quick Overview for NOV Protein (His tag) (ABIN7669349)
抗原
See all NOV 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This NOV protein is labelled with His tag.
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原理
- Recombinant Mouse NOV/CCN3 Protein(His Tag)
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序列
- Gln22-lle354
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纯化方法
- SDS-PAGE analysis of Mouse NOV/CCN3 proteins, 2 μg/lane of Recombinant Mouse NOV/CCN3 proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 50 KD
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU/mg of the protein as determined by the LAL method
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Biological Activity Comment
- Not validated for activity
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Nephroblastoma Overexpressed (NOV) (AA 1-357) protein (GST tag)
NOV 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nephroblastoma Overexpressed (NOV) (AA 28-357) protein (His-IF2DI Tag)NOV 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Nephroblastoma Overexpressed (NOV) (AA 26-354) protein (His tag)NOV 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- NOV (Nephroblastoma Overexpressed (NOV))
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别名
- NOV
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背景
- CCN,CCN family member,NovH,CCN family member 3,Nephroblastoma-overexpressed gene protein homolog,Protein NOV homolog,Nov,Ccn3,Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is a putative ligand for integrin receptors, is tightly associated with the extracellular matrix, and mediates diverse cellular functions, including cell adhesion and proliferation. CCN3 has been shown to negatively regulate growth althoμgh it promotes migration in a cell type-specific manner. This secreted protein belongs to the CCN family, and its expression was observed in a broad variety of tissues from the early stage of development, and altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm's tumors, Ewing's sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas. Mature CCN3 protein has five distinct modules and truncated protein variants with altered function are found in many cancers. CCN3 acts throμgh the core stem cell signaling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of some cell lineages including hematopoietic, osteogenic, and chondrogenic. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells. CCN3 has emerged as a key player in stem cell regulation, hematopoiesis, and a crucial component within the bone marrow microenvironment.
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分子量
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Calculated MW: 36.5 kDa
Observed MW: 50 kDa
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UniProt
- Q64299
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途径
- Smooth Muscle Cell Migration, Growth Factor Binding
抗原
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