LTA Protein (His tag)
Quick Overview for LTA Protein (His tag) (ABIN7669249)
抗原
See all LTA 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
-
-
标记
- This LTA protein is labelled with His tag.
-
原理
- Recombinant Mouse TNF beta protein(His Tag)
-
序列
- Leu 34-Leu 202
-
纯化方法
- > 98 % as determined by reducing SDS-PAGE.
-
过滤
- 0.2 μm filtered
-
内毒素水平
- < 0.1 EU per μg of the protein as determined by the LAL method.
-
Biological Activity Comment
- Measure by its ability to induce cytotoxicity in L929 cells in the presence of vactinomycin D. The ED50 for this effect is <30 ng/mL.
-
-
Lymphotoxin-alpha (LTA) (Active) protein
LTA 宿主: 化学剂 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Lymphotoxin-alpha (LTA) (AA 58-205) protein (His-DYKDDDDK-Strep II Tag)LTA 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Lymphotoxin-alpha (LTA) (AA 35-205) protein (His tag)LTA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Lymphotoxin-alpha (LTA) (AA 34-202) protein (His tag)LTA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Lymphotoxin-alpha (LTA) (AA 1-205) protein (GST tag)LTA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Lymphotoxin-alpha (LTA) (AA 35-205) (Active) protein (His tag)LTA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS, AcA Active
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
缓冲液
-
Lyophilized from sterile PBS, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
有效期
- 12 months
-
-
- LTA (Lymphotoxin-alpha (LTA))
-
别名
- TNF beta
-
背景
- Tumor Necrosis Factor β (TNF-β) is a secreted protein belonging to the tumor necrosis factor family. TNF-β binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM in homotrimeric form, binds to TNFRSF3/LTBR in heterotrimeric form with LTB. TNF-β forms heterotrimers with lymphotoxin-beta, which anchors TNF-β to the cell surface. TNF-β mediates the inflammatory, immunostimulatory, and antiviral response, involves in the formation of second lymphoid organs during development, has a role in apoptosis. TNF-β is produced by lymphocytes and cytotoxic for a variety of tumor cells in vitro and in vivo.,IFL,IFL-1,IFN-λ3,INF-alpha,INF-l,INF-lambda
-
分子量
-
calculated_mw: 19.4 kDa
observed_mw: 11-17 kDa
-
UniProt
- P09225
-
途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
-
