HFM1 Protein (AA 5325-5925) (His-MBP)
Quick Overview for HFM1 Protein (AA 5325-5925) (His-MBP) (ABIN7668895)
抗原
蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 5325-5925
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标记
- This HFM1 protein is labelled with His-MBP.
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原理
- Recombinant SARS-CoV-2 Helicase Protein (His Tag)
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序列
- Ala5325-Gln5925
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纯化方法
- > 80 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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HFM1, ATP-Dependent DNA Helicase Homolog (HFM1) (AA 1-1434) protein (His tag)
custom-made HFM1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
HFM1, ATP-Dependent DNA Helicase Homolog (HFM1) (AA 1-1435) protein (His tag)custom-made HFM1 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- HFM1 (HFM1, ATP-Dependent DNA Helicase Homolog (HFM1))
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别名
- Helicase
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背景
- The non-structural protein 13 (nsp13) of SARS-CoV 2 is a helicase that separates double-stranded RNA or DNA with a 5'-3' polarity, using the energy of nucleotide hydrolysis. A basic biochemical characterization of nsp13 demonstrated that it can unwind both doublestranded DNA and RNA in a 5'-3' direction, and it can hydrolyze all deoxyribonucleotide and ribonucleotide triphosphates. Helicases are motor proteins that utilize the energy derived from nucleotide hydrolysisto unwind double-stranded nucleic acids into two single-stranded nucleic acids. Initially, helicases were only thought to be molecular engines that unwind nucleic acids during replication, recombination, and DNA repair. Recent studies have shown that they are also involved in other biological processes, including displacement of proteins from nucleic acid, movement of Holliday junctions, chromatin remodeling, catalysis of nucleic acid conformational changes, several aspects of RNA metabolism, including transcription, mRNA splicing, mRNA export, translation, RNA stability and mitochondrial gene expression. Some human diseases, including Bloom's syndrome, Werner's syndrome, and Xeroderma Pigmentosum have been associated with defects in helicase function.,SARS-CoV 2 Helicase,SARS-CoV 2 nsp13
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分子量
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calculated_mw: 112.8 kDa
observed_mw: 120 kDa
抗原
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