Endoglin Protein (ENG) (His tag)
Quick Overview for Endoglin Protein (ENG) (His tag) (ABIN7668670)
抗原
See all Endoglin (ENG) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This Endoglin protein is labelled with His tag.
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原理
- Recombinant Mouse Endoglin/CD105 protein (His tag)
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序列
- Ser 431-Ala 653
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过滤
- 0.2 μm filtered
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内毒素水平
- < 10 EU/mg of the protein as determined by the LAL method.
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Endoglin (ENG) (AA 26-586) protein (His-Avi Tag)
ENG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Endoglin (ENG) (AA 1-658) protein (GST tag)ENG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Endoglin (ENG) (AA 394-581) protein (His-IF2DI Tag)ENG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Endoglin (ENG) (AA 27-658) protein (GST tag)ENG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Endoglin (ENG) (AA 26-586) protein (His tag)ENG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85% by SDS-PAGE SDS
Endoglin (ENG) (AA 27-581) (Active) protein (His tag)ENG 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS, AcA Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Endoglin (ENG)
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别名
- Endoglin,CD105
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背景
- Endoglin, also known as CD105, is a type I , homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type I ,I receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.,AI528660,AI662476,CD105,Endo,S-endoglin
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分子量
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calculated_mw: 24.4 kDa
observed_mw: 30 kDa
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UniProt
- Q63961
抗原
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