Coronavirus Spike Glycoprotein Protein (CoV S) (AA 14-685)
This Recombinant Coronavirus Spike Glycoprotein protein is expressed in HEK-293 Cells.
Quick Overview for Coronavirus Spike Glycoprotein Protein (CoV S) (AA 14-685) (ABIN7668597)
抗原
See all Coronavirus Spike Glycoprotein (CoV S) 蛋白
Coronavirus Spike Glycoprotein (CoV S)
蛋白类型
Recombinant
生物活性
Active
宿主
SARS Coronavirus-2 (SARS-CoV-2)
资源
HEK-293 Cells
应用范围
ELISA
纯度
> 95 %
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产品特性
- AA 14-685
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原理
- Recombinant SARS-CoV-2 S1 Protein
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序列
- Gln14-Arg685
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纯化方法
- > 95 % as determined by reducing SDS-PAGE.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Immobilized Human ACE-2-Fc(Cat#PKSR030492) at 10μg/ml (100 μl/well) can bind Recombinant 2019-nCoV S1 Protein (Cat#PKSR030481). Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant 2019-nCoV S1 Protein (Cat#PKSR030481) is 1. 18 ug/ml.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Coronavirus Spike Glycoprotein (CoV S)
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别名
- Spike
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物质类
- Viral Protein
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背景
- The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2, DPP4, dipeptidyl peptidase-4, APN, aminopeptidase N, CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1, Sia, sialic acid, O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion, Defines the range of the hosts and specificity of the virus, Main component to bind with the neutralizing antibody, Key target for vaccine design, Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.,2019-nCoV S1 protein,S1 protein,coronavirus S1 Protein,cov S1 Protein
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分子量
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calculated_mw: 78.3 kDa
observed_mw: 100-130 kDa
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UniProt
- P0DTC2
抗原
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