Cathepsin B Protein (CTSB) (His tag)
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Quick Overview for Cathepsin B Protein (CTSB) (His tag) (ABIN7668323)
抗原
See all Cathepsin B (CTSB) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Cathepsin B protein is labelled with His tag.
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原理
- Recombinant Human Cathepsin B Protein(His Tag)
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序列
- Met1-lle339
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纯化方法
- SDS-PAGE analysis of Human Cathepsin B proteins, 2μg/lane of Recombinant Human Cathepsin B proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 38-42 kDa
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU/mg of the protein as determined by the LAL method
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Biological Activity Comment
- Not validated for activity
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Cathepsin B (CTSB) (AA 21-339) protein (GST tag)
CTSB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cathepsin B (CTSB) (AA 1-339) protein (GST tag)CTSB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cathepsin B (CTSB) (AA 18-339) (Active) protein (His tag)CTSB 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS, EAA Active
Cathepsin B (CTSB) (AA 18-339) protein (His tag)CTSB 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % SDS
Cathepsin B (CTSB) (Active) protein (His tag)CTSB 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Cathepsin B (CTSB) (AA 18-339) protein (His tag)CTSB 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Cathepsin B (CTSB) (AA 1-339) protein (His tag)CTSB 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Cathepsin B (CTSB) (AA 82-333), (partial) protein (GST tag)CTSB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Cathepsin B (CTSB) (AA 18-339) protein (His tag)CTSB 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Cathepsin B (CTSB)
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别名
- Cathepsin B
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背景
- APPS,CPSB,Cathepsin B,CTSB,APP Secretase,CathepsinB,CATB,Cathepsin B1,cysteine protease,Amyloid precursor protein secretase,Cathepsin B heavy chain,OTTHUMP00000116009,OTTHUMP00000229510,OTTHUMP00000229511,OTTHUMP00000229512,OTTHUMP00000229514,OTTHUMP00000229515,OTTHUMP00000229516,Preprocathepsin B,Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.
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分子量
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Calculated MW: 37.2 kDa
Observed MW: 38-42 kDa
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基因ID
- 1508
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UniProt
- P07858
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途径
- Activation of Innate immune Response, Toll-Like Receptors Cascades
抗原
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