GRIK3 Protein (DYKDDDDK Tag,Strep Tag)
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Quick Overview for GRIK3 Protein (DYKDDDDK Tag,Strep Tag) (ABIN7597215)
抗原
See all GRIK3 蛋白蛋白类型
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资源
应用范围
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标记
- This GRIK3 protein is labelled with DYKDDDDK Tag,Strep Tag.
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原理
- Human GRIK3-Strep full length protein-synthetic nanodisc
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产品特性
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Advantages:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please see Certificate of Analysis for specific instructions of reconstitution
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缓冲液
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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有效期
- 12 months
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- GRIK3 (Glutamate Receptor, Ionotropic, Kainate 3 (GRIK3))
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别名
- GRIK3
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背景
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EAA5, GLR7, GLUR7, GluK3, GluR7a
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. It is not certain if the subunit encoded by this gene is subject to RNA editing as the other 2 family members (GRIK1 and GRIK2). A Ser310Ala polymorphism has been associated with schizophrenia, and there are conflicting reports of its association with the pathogenesis of delirium tremens in alcoholics. [provided by RefSeq, Jul 2008] -
分子量
- The human full length GRIK3-Strep protein has a MW of 104 kDa
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UniProt
- Q13003
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途径
- Synaptic Membrane
抗原
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