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GRIK3 Protein (DYKDDDDK Tag,Strep Tag)

This Synthetic Nanodisc GRIK3 protein is expressed in HEK-293 Cells.
产品编号 ABIN7597215
发货至: 中国
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Quick Overview for GRIK3 Protein (DYKDDDDK Tag,Strep Tag) (ABIN7597215)

抗原

See all GRIK3 蛋白
GRIK3 (Glutamate Receptor, Ionotropic, Kainate 3 (GRIK3))

蛋白类型

Synthetic Nanodisc

宿主

资源

  • 2
HEK-293 Cells

应用范围

ELISA, Cryogenic electron microscopy (cryo-EM), Immunogen (Imm), Phage Display (PhD), Surface Plasmon Resonance (SPR)
  • 标记

    This GRIK3 protein is labelled with DYKDDDDK Tag,Strep Tag.

    原理

    Human GRIK3-Strep full length protein-synthetic nanodisc

    产品特性

    Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    Advantages:

    • Highly purified membrane proteins
    • High solubility in aqueous solutions
    • High stability
    • Proteins are in a native membrane environment and remain biologically active
    • No detergent and can be used for cell-based assays
    • No MSP backbone proteins
    Limitations:
    • Intolerant to acids and high concentrations of divalent metal ions

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Please see Certificate of Analysis for specific instructions of reconstitution

    缓冲液

    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

    储存条件

    -20 °C,-80 °C

    储存方法

    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

    有效期

    12 months
  • 抗原

    GRIK3 (Glutamate Receptor, Ionotropic, Kainate 3 (GRIK3))

    别名

    GRIK3

    背景

    EAA5, GLR7, GLUR7, GluK3, GluR7a
    Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. It is not certain if the subunit encoded by this gene is subject to RNA editing as the other 2 family members (GRIK1 and GRIK2). A Ser310Ala polymorphism has been associated with schizophrenia, and there are conflicting reports of its association with the pathogenesis of delirium tremens in alcoholics. [provided by RefSeq, Jul 2008]

    分子量

    The human full length GRIK3-Strep protein has a MW of 104 kDa

    UniProt

    Q13003

    途径

    Synaptic Membrane
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