CHRNG Protein (DYKDDDDK Tag,Strep Tag)
This Synthetic Nanodisc CHRNG protein is expressed in HEK-293 Cells.
产品编号 ABIN7597169
发货至:
中国
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Quick Overview for CHRNG Protein (DYKDDDDK Tag,Strep Tag) (ABIN7597169)
抗原
See all CHRNG 蛋白
CHRNG
(Cholinergic Receptor, Nicotinic, gamma (CHRNG))
蛋白类型
Synthetic Nanodisc
宿主
人
资源
HEK-293 Cells
应用范围
Immunogen (Imm), ELISA, Cryogenic electron microscopy (cryo-EM), Phage Display (PhD), Surface Plasmon Resonance (SPR)
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标记
- This CHRNG protein is labelled with DYKDDDDK Tag,Strep Tag.
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原理
- Human ACHG-Strep full length protein-synthetic nanodisc
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产品特性
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Advantages:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please see Certificate of Analysis for specific instructions of reconstitution
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缓冲液
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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有效期
- 12 months
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- CHRNG (Cholinergic Receptor, Nicotinic, gamma (CHRNG))
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别名
- ACHG
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背景
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ACHRG
The mammalian muscle-type acetylcholine receptor is a transmembrane pentameric glycoprotein with two alpha subunits, one beta, one delta, and one epsilon (in adult skeletal muscle) or gamma (in fetal and denervated muscle) subunit. This gene, which encodes the gamma subunit, is expressed prior to the thirty-third week of gestation in humans. The gamma subunit of the acetylcholine receptor plays a role in neuromuscular organogenesis and ligand binding and disruption of gamma subunit expression prevents the correct localization of the receptor in cell membranes. Mutations in this gene cause Escobar syndrome and a lethal form of multiple pterygium syndrome. Muscle-type acetylcholine receptor is the major antigen in the autoimmune disease myasthenia gravis.[provided by RefSeq, Sep 2009] -
分子量
- The human full length ACHG-Strep protein has a MW of 57.9 kDa
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UniProt
- P07510
抗原
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