SHKB1 protein (DYKDDDDK Tag,Strep Tag)
This Synthetic Nanodisc SHKB1 protein is expressed in HEK-293 Cells.
产品编号 ABIN7597077
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for SHKB1 protein (DYKDDDDK Tag,Strep Tag) (ABIN7597077)
抗原
SHKB1
蛋白类型
Synthetic Nanodisc
宿主
人
资源
HEK-293 Cells
应用范围
Cryogenic electron microscopy (cryo-EM), ELISA, Immunogen (Imm), Phage Display (PhD), Surface Plasmon Resonance (SPR)
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标记
- DYKDDDDK Tag,Strep Tag
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原理
- Human SHKB1-Strep full length protein-synthetic nanodisc
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产品特性
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Advantages:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please see Certificate of Analysis for specific instructions of reconstitution
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缓冲液
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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有效期
- 12 months
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- SHKB1
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背景
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PP203, Sb1
Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.[UniProtKB/Swiss-Prot Function] -
分子量
- The human full length SHKB1-Strep protein has a MW of 76.3 kDa
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UniProt
- Q8TBC3
抗原
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