ALDH1A1 Protein (AA 1-501)
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北京 101111
Quick Overview for ALDH1A1 Protein (AA 1-501) (ABIN7677887)
抗原
See all ALDH1A1 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 1-501
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序列
- MSSSGTPDLP VLLTDLKIQY TKIFINNEWH DSVSGKKFPV FNPATEEELC QVEEGDKEDV DKAVKAARQA FQIGSPWRTM DASERGRLLY KLADLIERDR LLLATMESMN GGKLYSNAYL NDLAGCIKTL RYCAGWADKI QGRTIPIDGN FFTYTRHEPI GVCGQIIPWN FPLVMLIWKI GPALSCGNTV VVKPAEQTPL TALHVASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDID KVAFTGSTEV GKLIKEAAGK SNLKRVTLEL GGKSPCIVLA DADLDNAVEF AHHGVFYHQG QCCIAASRIF VEESIYDEFV RRSVERAKKY ILGNPLTPGV TQGPQIDKEQ YDKILDLIES GKKEGAKLEC GGGPWGNKGY FVQPTVFSNV TDEMRIAKEE IFGPVQQIMK FKSLDDVIKR ANNTFYGLSA GVFTKDIDKA ITISSALQAG TVWVNCYGVV SAQCPFGGFK MSGNGRELGE YGFHEYTEVK TVTVKISQKN S
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纯化方法
- > 90% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole propioaldehyde by NAD per minute at pH 8.8 at 37°C.
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Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) (AA 1-501) protein (GST tag)
ALDH1A1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) (AA 2-501) protein (His-IF2DI Tag)ALDH1A1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) (AA 18-517) (Active) proteinALDH1A1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS, EAA Active
Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) (AA 1-501) proteinALDH1A1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 50 mM Tris-HCl buffer ( pH 7.5) containing 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))
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别名
- Aldehyde Dehydrogenase 1-A1/ ALDH1A1
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背景
- Aldehyde dehydrogenase 1A1 (ALDH1A1), also known as retinal dehydrogenase 1, is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea. (Retinal + NAD+ + H2O = retinoate + NADH) Recombinant ALDH1A1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 54.8 kDa (501aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_000680
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途径
- Dopaminergic Neurogenesis
抗原
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