IgG1 Fc (AA 100-330) (Active) 蛋白
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北京 101111
Quick Overview for IgG1 Fc (AA 100-330) (Active) 蛋白 (ABIN7677877)
抗原
蛋白类型
生物活性
宿主
资源
应用范围
纯度
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产品特性
- AA 100-330
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序列
- PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K
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纯化方法
- > 90% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- 1. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4102). The ED50 range ≤ 1.5 ug/ml., 2. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4116)., 3. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4098). The ED50 range ≤ 4 ug/ml.
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IgG1 Fc (AA 104-330) protein
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgG1 Fc (AA 98-324) protein宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgG1 Fc protein (Fc Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
IgG1 Fc protein宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. WB
IgG1 Fc (AA 100-330) protein (DYKDDDDK Tag)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- IgG1 Fc
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背景
- Immunoglobulins are membrane-bound or secreted glycoproteins produced by B lymphocytes. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors on phagocytic cells with high affinity. IgG comprise two identical light (L) and heavy (H) chains forming a 'Y-shaped' structure. It also is digested by pepsin proteolysis into two distinct fragments (Fab, Fc). Fc fragment is a homodimeric glycoprotein with one monomer consisting of two constant immunoglobulin domains (CH2, CH3) from the C-terminal half of one heavy chain. Through interaction with its ligands (FcγR, C1q and FcRn) it mediates various immune effector functions and increases the half-life of the antibody molecule. Recombinant IgG Fc is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases. Recombinant human IgG1 Fc was expressed in HEK293 cell and purified by using conventional chromatography techniques.
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分子量
- 25.9kDa (231aa)
抗原
-
