MSTN Protein (AA 267-375)
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北京 101111
Quick Overview for MSTN Protein (AA 267-375) (ABIN7583284)
抗原
See all MSTN 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 267-375
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原理
- Human/Mouse/Rat GDF-8 Protein
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序列
- Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat)
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产品特性
- Recombinant Human/Mouse/Rat GDF-8 Protein is expressed from HEK293 without tag.It contains Asp267-Ser375 (Human) / Asp268-Ser376 (Mouse/Rat).
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Human Activin RIIA, hFc Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).,Biotinylated Activin RIIB, His Tag-captured on CM5 Chip via Streptavidin can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).,Anti-GDF8 Antibody, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 49.45 pM as determined in SPR assay (Biacore T200).,Immobilized Human/Mouse/Rat GDF-8, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.,Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIA, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA.,Immobilized Human GDF-8, No Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 6.9 ng/ml determined by ELISA.,Human/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.,Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected-ED50-for this effect is <30 ng/ml (QC Test).
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Myostatin (MSTN) (Active) protein
MSTN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Myostatin (MSTN) (AA 24-375) protein (Fc Tag)MSTN 宿主: 人 宿主: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Myostatin (MSTN) (AA 267-375) protein (His-IF2DI Tag)MSTN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Myostatin (MSTN) (AA 1-375) protein (GST tag)MSTN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Myostatin (MSTN) proteinMSTN 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in 4 mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22 μm filtered solution in 4 mM HCL Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- MSTN (Myostatin (MSTN))
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别名
- GDF-8
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背景
- Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
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分子量
- 12.40 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.
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UniProt
- O14793
抗原
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