产品编号 ABIN7582362
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Taipoxin 蛋白 (ABIN7582362)
抗原
Taipoxin
蛋白类型
Native
宿主
Oxyuranus scutellatus scutellatus
资源
Snake (Serpentes)
应用范围
Blocking Peptide (BP), Immunocytochemistry (ICC)
纯度
≥97 % (HPLC)
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原理
- A Blocker of Synaptic Vesicle Recycling
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序列
- i±, i² and i³ chains non-covalently linkedS
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特异性
- Highly disulfide bridged protein, glycosylated
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产品特性
- A Blocker of Synaptic Vesicle Recycling
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应用备注
- Antigen Preadsorption Control: 10 - 50 nM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
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缓冲液
- Lyophilized from filtrated Ammonium acetate solution. May contain acetate as a residual counter ion.
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储存条件
- 4 °C,-20 °C
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储存方法
- The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
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- Taipoxin
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背景
- Taipan toxin,Taipoxin is a lethal neurotoxin protein isolated from the venom of the Australian taipan Oxyuranus s. scutellatus (LD50= 2 μg/kg in mouse). Taipoxin is a non-covalent ternary glycoprotein which is completely dissociated at low pH or high ionic strength. One of the subunits has Phospholipase A2 activity1,2.Intoxicated animals die of asphyxia caused by a complete inhibition of neurotransmitter release and neuromuscular junction blockage of the respiratory muscles. In the central nervous system taipoxin blocks synaptic vesicle recycling by inhibiting the neuronal uptake pathway through the interaction with neuronal Pentraxin system components during synapse formation and remodeling1,3-5.In neuronal cultures, Taipoxin facilitates Ca2+-dependent synaptic vesicle exocytosis and causes a complete depletion of stored neurotransmitter, resulting in synaptic transmission blockage. Taipoxin, at nanomolar range, causes swelling of nerve terminus and redistribution of synaptic vesicle proteins7.
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分子量
- 46 kDa
抗原
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