产品编号 ABIN7582345
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for MitTx Toxin 蛋白 (ABIN7582345)
抗原
MitTx Toxin
蛋白类型
Native
宿主
Micrurus tener tener
资源
Snake (Serpentes)
应用范围
Blocking Peptide (BP)
纯度
≥98 % (HPLC)
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原理
- An Activator of ASIC1-Containing Channels
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序列
- MitTx-i± subunit:QIRPAFCYED PPFFQKCGAF VDSYYFNRSR ITCVHFFYGQ CDVNQNHFTT MSECNRVCHG MitTx-i² subunit:NLNQFRLMIK CTNDRVWADF VDYGCYCVAR DSNTPVDDLD RCCQAQKQCY DEAVKVHGCK PLVMFYSFEC RYLASDLDCS GNNTKCRNFV CNCDRTATLC ILTATYNRNN HKIDPSRCQ
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特异性
- Toxin target: ASIC1 channels, MitTx-i± subunit contains pyrrolidone carboxylic acid at the N-terminus.
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产品特性
- An Activator of ASIC1-Containing Channels
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应用备注
- Antigen Preadsorption Control: 20 nM
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说明
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Electrophysiology
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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储存条件
- 4 °C,-20 °C
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储存方法
- The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
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- MitTx Toxin
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背景
- MitTx, alpha/beta MitTx,alpha/beta MitTx (MitTx toxin) is a natural toxin isolated from the Texas Coral snake venom1. The purified active toxin consists of a heteromeric complex between Kunitz (α subunit) and phospholipase-A2-like (β subunit) proteins that together function as a potent, persistent and selective agonist for acid-sensing ion channels (ASICs), showing equal or greater efficacy compared with acidic pH 1.MitTx-α and MitTx-β form a high affinity 1:1 complex, and neither subunit demonstrates functional effects on its own1,2.The purified ASIC-activating component of the venom, MitTx, was found to be sufficient to elicit robust nocifensive behaviors and is likely responsible for the lasting pain experienced by victims of coral snake envenomation2.
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分子量
- 20.84 kDa (i± subunit, 7.104 kDa and i² subunit, 13.736 kDa)
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UniProt
- G9I930
抗原
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