kappa-Bungarotoxin 蛋白
This Native kappa-Bungarotoxin protein is expressed in Snake (Serpentes).
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产品编号 ABIN7582319
发货至:
中国
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Quick Overview for kappa-Bungarotoxin 蛋白 (ABIN7582319)
抗原
kappa-Bungarotoxin
蛋白类型
Native
宿主
Bungarus multicinctus
资源
Snake (Serpentes)
应用范围
Blocking Peptide (BP)
纯度
≥98 % (HPLC)
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原理
- A Potent Antagonist of α3/β2 Neuronal nAChR
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序列
- RTCLISPSST PQTCPNGQDI CFLKAQCDKF CSIRGPVIEQ GCVATCPQFR SNYRSLLCCT TDNCNH-OH
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特异性
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Toxin target: α3/β2 Neuronal nAChR,
Disulfide bonds between: Cys3-Cys21, Cys14-Cys42, Cys27-Cys31, Cys46-Cys58, Cys59-Cys64 -
产品特性
- A Potent Antagonist of α3/β2 Neuronal nAChR
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应用备注
- Antigen Preadsorption Control: 5 - 25 nM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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储存条件
- 4 °C,-20 °C
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储存方法
- The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
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- kappa-Bungarotoxin
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背景
- Kappa-Bungarotoxin, Kappa-bgt, Kappa-1-bungarotoxin, Long neurotoxin 2,Neuronal bungarotoxin, nBgt, Toxin F,Kappa-bungarotoxin (κ-Bungarotoxin, κ-Bgt) is a 66 amino acid peptidyl toxin isolated from the venom of the banded krait snake, Bungarus multicinctus1,2. κ-Bgt is a postsynaptic neurotoxin that potently binds and inhibits the neuronal types of nicotinic acetylcholine receptors (nAChR). It is a selective antagonist of α3-containing and some α4-containing nAChRs subunits3.Under physiological solvent conditions, κ-Bungarotoxin exists as a non-covalent homodimer4. The dimeric nature of the toxin may play a crucial role in the binding properties of κ-Bgt to neuronal types of AChRs while losing its potency on muscle receptors4-6.Neuronal nicotinic acetylcholine receptors (nAChRs) are prototypical cation-selective, ligand-gated ion channels that mediate fast neurotransmission in the central and peripheral nervous systems. nAChRs are involved in a range of physiological and pathological functions and hence are important therapeutic targets7. The κ-neurotoxins have been valuable pharmacological tools in the study of neuronal AChRs3.
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分子量
- 7265 Da
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UniProt
- P01398
抗原
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