alpha-Bungarotoxin (Active) protein (Atto 550)
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北京 101111
Quick Overview for alpha-Bungarotoxin (Active) protein (Atto 550) (ABIN7582314)
抗原
蛋白类型
生物活性
宿主
资源
应用范围
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标记
- Atto 550
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原理
- α-Bungarotoxin-ATTO Fluor-550
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序列
- IVCHTTATSP ISAVTCPPGE NLCYRKMWCD AFCSSRGKVV ELGCAATCPS KKPYEEVTCC STDKCNPHPK QRPG
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特异性
- Toxin target: α1/β1/γ/δ, α7 nAChR, GABA(A) receptor subtypes
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产品特性
- ATTO 550 (Ex/Em: 554/576 nm). The extent of labeling is 1-3 Molecules of dye per molecule α-Bungarotoxin, linked via primary amines.
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Biological Activity Comment
- α-Bungarotoxin blocks postsynaptic neuromuscular transmission via competitive inhibition of nicotinic ACh receptors (nAChRs).
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alpha-Bungarotoxin (Active) protein (Atto 647N)
宿主: Bungarus multicinctus 宿主: Bungarus multicinctus (Many-banded krait) Native FM, FACS, IF, LCI Active
alpha-Bungarotoxin (Active) protein (Atto 633)宿主: Bungarus multicinctus 宿主: Bungarus multicinctus (Many-banded krait) Native FM, FACS, IF, LCI Active
alpha-Bungarotoxin (Active) protein (Atto 488)宿主: Bungarus multicinctus 宿主: Bungarus multicinctus (Many-banded krait) Native FM, FACS Active
alpha-Bungarotoxin (Active) protein (Atto 590)宿主: Bungarus multicinctus 宿主: Bungarus multicinctus (Many-banded krait) Native FM, FACS, IF, LCI Active
alpha-Bungarotoxin (Active) protein (Biotin)宿主: Bungarus multicinctus 宿主: Bungarus multicinctus (Many-banded krait) Native FACS, IF Active
alpha-Bungarotoxin (Active) protein (FITC)宿主: Bungarus multicinctus 宿主: Bungarus multicinctus (Many-banded krait) Native FM Active
alpha-Bungarotoxin (Active) protein宿主: Bungarus multicinctus 宿主: Bungarus multicinctus (Many-banded krait) Native ≥99 % (HPLC) Active
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应用备注
- Antigen Preadsorption Control: 0.1 - 0.5 μM
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Note: The product is typically supplied in a 0.5 mL conical vial. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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注意事项
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light
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储存条件
- -20 °C
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储存方法
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.
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- alpha-Bungarotoxin
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背景
- A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
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分子量
- ~9134 Da
抗原
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