Quick Overview for alpha-Bungarotoxin 蛋白 (ABIN7582311)
抗原
蛋白类型
宿主
资源
应用范围
纯度
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原理
- A Potent Antagonist of α7 and Muscle nAChR and GABA(A) Receptor Subtypes
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序列
- IVCHTTATSP ISAVTCPPGE NLCYRKMWCD AFCSSRGKVV ELGCAATCPS KKPYEEVTCC STDKCNPHPK QRPG-OH
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特异性
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Toxin target: α7, α1/β1/γ/δ nAChR, GABA(A) receptor subtypes,
Disulfide bonds between: Cys3-Cys23, Cys16-Cys44, Cys29-Cys33, Cys48-Cys59 and Cys60-Cys65 -
产品特性
- A Potent Antagonist of α7 and Muscle nAChR and GABA(A) Receptor Subtypes
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alpha-Bungarotoxin protein (Atto 647N)
宿主: Bungarus multicinctus 宿主: Snake (Serpentes) Native BP, FACS, FM, IF, LCI
alpha-Bungarotoxin protein (Atto 633)宿主: Bungarus multicinctus 宿主: Snake (Serpentes) Native BP, FACS, FM, IF, LCI
alpha-Bungarotoxin protein (Atto 488)宿主: Bungarus multicinctus 宿主: Snake (Serpentes) Native BP, FACS, FM
alpha-Bungarotoxin protein (Biotin)宿主: Bungarus multicinctus 宿主: Snake (Serpentes) Native BP, FACS, IF
alpha-Bungarotoxin protein (Atto 590)宿主: Bungarus multicinctus 宿主: Snake (Serpentes) Native BP, FACS, FM, IF, LCI
alpha-Bungarotoxin protein (Atto 550)宿主: Bungarus multicinctus 宿主: Snake (Serpentes) Native BP, FACS, FM, IF, LCI
alpha-Bungarotoxin protein (FITC)宿主: Bungarus multicinctus 宿主: Snake (Serpentes) Native BP, FM
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应用备注
- Antigen Preadsorption Control: 1 nM - 3 μM
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说明
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Electrophysiology
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
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缓冲液
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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储存条件
- 4 °C,-20 °C
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储存方法
- The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
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- alpha-Bungarotoxin
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背景
- Long neurotoxin 1, α-Bgtx, α-BuTX,α-Bungarotoxin isoform A31 is a 74 amino acid peptidyl toxin isolated from the venom of the banded krait snake, Bungarus multicinctus1.α-Bungarotoxin blocks postsynaptic neuromuscular transmission via competitive inhibition of nicotinic ACh receptors (nAChRs) with an IC50 of 3.5 x 10-10 M, thereby preventing the depolarizing action on postsynaptic membranes and blocking neuromuscular transmission2.The toxin is selective for α7 receptors (IC50 value of 1.6 nM) and α3/β4 receptors (IC50 value of >3 μM)3,4.α-Bungarotoxin also binds to and blocks a subset of GABAA receptors (GABAARs) that contain the GABAAR β3 subunit. In particular, α-Bungarotoxin blocks GABAARs that contain interfaces between adjacent β3 subunits5.
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分子量
- 7984 Da
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UniProt
- P60615
抗原
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