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- 抗原
- PolyStreptavidin
- 蛋白类型
- Native
- 宿主
- Streptomyces avidinii
- 资源
- Streptomyces avidinii
- 应用范围
- Functional Studies (Func), Lateral Flow (LF)
- 原理
- Polymerized Streptavidin
- 产品特性
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Polystreptavidin is a chemically modified, polymerized form of Streptavidin distinguished by its exceptionally high biotin-binding capacity. When applied as a coating on solid surfaces, Polystreptavidin provides a versatile platform for immobilizing and detecting a wide range of biotinylated molecules—including proteins, peptides, PCR fragments, haptens, and more.
These coatings combine outstanding binding efficiency with excellent chemical and thermal stability, along with an extended shelf life. Polystreptavidin is ideal for use on various substrates such as membranes, beads, biochips, and plastic materials, offering a robust and reliable solution for diverse analytical and diagnostic applications. - 纯化方法
- Dialysis
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- 应用备注
- Lateral Flow Assay (LFA): 2 mg/mL in PBS
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 5 mg/mL
- 缓冲液
- 5 mM PBS, 0.09% Sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- 2 to 8 °C. Do not freeze!
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- 抗原
- PolyStreptavidin
- 背景
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Streptavidin is a tetrameric protein, composed of four subunits of approximately 13 kDa each, that exhibits an exceptionally strong affinity for the small molecule biotin. This interaction ranks among the most robust non-covalent bonds in biology and remains stable under extreme conditions such as high temperatures, varying pH levels, and exposure to solvents or other chemicals. Biotin can effectively outcompete most other ligands for streptavidin binding and can be easily linked to a variety of biomolecules, making this system highly versatile and widely used—especially in immunoassay applications.
A single streptavidin molecule is capable of binding up to four biotin or biotinylated proteins. However, the use of Polystreptavidin allows for the attachment of significantly more biotin molecules per complex. This enhanced binding capacity can lead to lower background noise, improved signal strength and specificity, and reduced reagent costs during the development and optimization of immunoassays.
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