Interleukin 35 Protein (IL35) (AA 23-215, AA 23-228) (Fc Tag)
Quick Overview for Interleukin 35 Protein (IL35) (AA 23-215, AA 23-228) (Fc Tag) (ABIN7566267)
抗原
See all Interleukin 35 (IL35) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 23-215, AA 23-228
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标记
- This Interleukin 35 protein is labelled with Fc Tag.
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原理
- IL-35 (mouse):Fc (human) (rec.)
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交叉反应
- 小鼠
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产品特性
- A soluble dimeric fusion protein consisting of the extracellular domain of mouse IL12a subunit (aa 23-215) is fused to the Fc region of human IgG1, and the mouse Ebi3 subunit (aa 23-228) linked to IL12a by disulfide bonds.
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过滤
- Sterile filtered
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内毒素水平
- <1EU/mg protein (LAL test, Lonza).
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Biological Activity Comment
- Bioactivity was measured in a cell proliferation assay of Con A activated mouse splenocytes.
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Interleukin 35 (IL35) (AA 21-229), (AA 23-219) (Active) protein (His tag)
IL35 宿主: 人 宿主: 大肠杆菌（E. Coli） > 97 % AcA, CC Active
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Lyophilized from 0.2μm-filtered solution in PBS.
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注意事项
- Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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- Interleukin 35 (IL35)
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别名
- IL-35
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背景
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Interleukin-35
Interleukin-35 (IL-35) is a novel IL-12 family cytokine produced by regulatory T cells (Treg) but not by resting or activated effector T cells (Teff). IL-35 is a heterodimeric protein composed of EBI3 (Epstein-Barr-Virus-induced gene 3) and IL-12a (p35). EBI3 is a downstream target of Foxp3, a transcription factor required for Treg-cell development and function, and thus Treg-cell restriction of IL35 occurs. Regulatory T cells are essential for maintaining self tolerance and preventing autoimmunity, and IL-35 is identified as a molecule that mediates the immune suppression function of Treg-cell. As an inhibitory cytokine, IL-35 induces proliferation of Treg-cell populations but suppresses Th17 cell development. Studies in mice show the absence of either IL-35 chain from Treg-cell reduces the cells' ability to suppress inflammation using an experimental model for inflammatory bowel disease. IL-35 is suggested as a potential target of immunotherapy. Recently, insufficient IL-35 levels were shown to play a pivotal role in the development of type 1 diabetes (T1D) and autoimmune diseases.
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分子量
- ~30-60kDa
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NCBI登录号
- NP_032377
抗原
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