Quick Overview for Cingulin Protein (CGN) (AA 1-1191) (His tag) (ABIN7563478)
抗原
Cingulin (CGN)
蛋白类型
Recombinant
宿主
小鼠
资源
HEK-293 Cells
纯度
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
质量等级
custom-made
产品特性
AA 1-1191
标记
This Cingulin protein is labelled with His tag.
原理
Predefined custom protein recombinant Cgn Protein expressed in mammalian cells.
序列
MADPRGPVDH GVQIRFITEP EGATEMGTLR RSGRRPARDA RASTYGVAVR VQGIAGQPFV VLNSGEKGTD SFGVQIKGGN NRGSPGALSS DSELPENPYS QVKGRPATSR SSTSDEEPKD HLNGKLIRSQ SQASLTGLAF MSPSNRSTSL LELAPKPTSS INTIDTAPLS SVDSLINKFD SQKGGQVRGR TGRRTRTLPH EQRKRSQSLD SRLPRDTREE REHQSANHWT RGTKYDNHVD SSKNPSQKQS PFSSFSRSRQ TQDWVLQSFE ETRDPAMVQF KSTPDLLRDQ RETAPPGSAD HVKATIYGIL REGSSESEAS VRRKVSLVLE QMQPLGMVSP ASTKALAGQA ELTRKMEELQ KKLDEEVKKR QKLEPSRVGL ERQLEEKAEE CHRLQELLER RKGEVQQSSK ELQNMKLLLG QEEGLRHGLE AQVKELQLKL KHSQSPDSGK ESLLKDLLDT RELLEELLEG KQRVEEQLRL RERELTALKG ALKEEVASHD QEVEHVRLQY QRDTEQLRRS MQDATQDHAA LEAERQKMSS LVRELQRELE ETSEETGHWQ SMFQKNKEEL RATKQELLQL RMEKEEMEEE LGEKMEVLQR DLEQARASTR DTHQVEELKK ELRRTQGELK ELQAEQQNQE VTGRHRNQVL EKQLAALREE ADRGRELEQQ NLQLQKTLQQ LRQDCEEASK AKVASETEAM MLGQRRATVE TTLRETQEEN DEFRRRILGL EQQLKEARGL AEGGEAVEAR LRDKVHRLEV EKQQLEEALN AAQEEEGNLA AAKRALEVRL DEAQRGLARL GQEQQALNRA LEEEGKQREA LRRSKAELEE QKRLLNRTVD RLNKELEQIG DDSKLALQQL QAQMEDYKEK ARKEVADAQR QAKDWASEAE KNSGGLSRLQ DELQRLRQAL QTSQAERDTA RLDKELLAQR LQGLEQEAEN KKRFQDDKAR QLKSLEEKVS RLEAELDEEK NTVELLTDRV NRGRDQVDQL RTELMQERSA RQDLECDKIS LERQNKDLKT RLASSEGFQK PSASLSQLES QNQLLQERLQ AEEREKTVLQ STNRKLERRV KELSIQIDDE RQHVNDQKDQ LTLRVKALKR QVDEAEEEIE RLDSLRKKAQ RELEEQHEVN EQLQARIKSL EKDAWRKASR SAAESALKQE GLSSDEEFDN VYDPSSIASL LTESNLQTSS C Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Predefined custom protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
custom-made
CGN
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
注意事项
Avoid repeated freeze-thaw cycles.
储存条件
-80 °C
储存方法
Store at -80°C.
有效期
12 months
抗原
Cingulin (CGN)
别名
Cgn
背景
Cingulin,FUNCTION: Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. {ECO:0000250}.