PKMYT1 Protein (AA 1-490) (His tag)
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北京 101111
Quick Overview for PKMYT1 Protein (AA 1-490) (His tag) (ABIN7561468)
抗原
See all PKMYT1 蛋白蛋白类型
宿主
资源
纯度
质量等级
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产品特性
- AA 1-490
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标记
- This PKMYT1 protein is labelled with His tag.
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原理
- Predefined custom protein recombinant Pkmyt1 Protein expressed in mammalian cells.
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序列
- MTMPTEGTPP PLSGTPIPVP AYFRHAEPGF SLKRPGGLSR SLPPRPPAKG CIPVSRLFPP RTPGWHQPQP RRVSFLCETS EPLQSPGYDP SRPESFFQQN FQRLSRLGHG SYGEVFKVRS KEDGRLYAVK RYMSPFRGPK DRTRKLAEVG GHEKVGQHPH CVRLERAWEE GGILYLQTEL CGPSLQQHCE AWGASLPEAQ VWGYLRDILL ALDHLHSQGL VHLDVKPANI FLGPRGRCKL GDFGLLVELG SAGAGEAQEG DPRYMAPELL QGSYGTAADV FSLGLTILEV ACNMELPHGG EGWQQLRQGY LPPEFTAGLS SELRSVLAMM LEPDPQLRAT AEALLALPML RQPRPWNVLW YMAAEALSRG WALWQALVTL LCWLWHGLVH PASWLQPPGP PATPPGSPPC SPLLDSTLSS SWDNDSIGPS LSPETVLSRI TRRTSTPRGR YIPRDALDLT DVDSEPPRGP CPTFEPRNLL SLFEDSLDPA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
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特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
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产品特性
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
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Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) (AA 1-499) protein (His tag)
custom-made PKMYT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) (AA 400-499) protein (GST tag)PKMYT1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PKMYT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- The buffer composition is at the discretion of the manufacturer.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -80 °C
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储存方法
- Store at -80°C.
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有效期
- 12 months
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- PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))
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别名
- Pkmyt1
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背景
- Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase (EC 2.7.11.1) (Myt1 kinase),FUNCTION: Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. {ECO:0000250|UniProtKB:Q99640}.
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分子量
- 54.1 kDa
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UniProt
- Q9ESG9
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AlphaFold
- Q9ESG9
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途径
- Mitotic G1-G1/S Phases, M Phase
抗原
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