> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
质量等级
custom-made
产品特性
AA 1-352
标记
This NUDT13 protein is labelled with His tag.
原理
Predefined custom protein recombinant Nudt13 Protein expressed in mammalian cells.
序列
MSLYCRTFFR RKSFGCYRLL STYVTKARYL FELKEDEEAC RKAQKTGVFY LFHDLDPLLQ ASGHRYLVPR LSRAELEGLL GKFGQDSQRI EDSVLVGCSE QQEAWFALDL GLKSASSSRA SLPKSEMEAE LGGSFIKLRQ ALFQLNSVDS SLLFTAQALL RWHDGHQFCS KSGQPTQKNV AGSKRVCPSS KIIYYPQMAP VVITLVSDGA RCLLARQSSF PKGLYSALAG FCDIGESVEE TVHREVAEEV GLEVENIQYS ASQHWPFPNS SLMIACHATV KPGHTEIQVN LKELEAAAWF SLDEVTTALR RKGSLALQPS EASPLLLPPK LAIAHHLIKK WVETRSCSSL AA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
特异性
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
产品特性
Key Benefits:
Predefined custom protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
custom-made
NUDT13
宿主: 小鼠
宿主: Cell-free protein synthesis (CFPS)
Recombinant
approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
custom-made
NUDT13
宿主: 人
宿主: Cell-free protein synthesis (CFPS)
Recombinant
approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, WB, SDS
NUDT13
宿主: 人
宿主: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
应用备注
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
限制
仅限研究用
状态
Liquid
缓冲液
The buffer composition is at the discretion of the manufacturer.
NAD(P)H pyrophosphatase NUDT13, mitochondrial (EC 3.6.1.22) (Nucleoside diphosphate-linked moiety X motif 13) (Nudix motif 13),FUNCTION: NAD(P)H pyrophosphatase that hydrolyzes NADH into NMNH and AMP, and NADPH into NMNH and 2',5'-ADP (PubMed:28755312). Has a marked preference for the reduced pyridine nucleotides (PubMed:28755312). Does not show activity toward NAD-capped RNAs, the NAD-cap is an atypical cap present at the 5'-end of some RNAs (PubMed:31101919). {ECO:0000269|PubMed:28755312, ECO:0000269|PubMed:31101919}.