Ube2fb (AA 1-185) protein (His tag)
custom-made
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- 抗原
- Ube2fb
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-185
- 宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- His tag
- 原理
- Custom-made recombinant Ube2fb Protein expressed in mammalian cells.
- 序列
- MLTLASKLKW DEDLKGSQTS VSTSDSTRRF SMRDKLLVTE VAELEANLPW TCKVHFPDPN KLHCFQLTVS PDEGYYQGGN FQFEIEVPDA YNMVPPKVKC LTKIWHPNIT ETGEICLSLL REHSADGTGW APTRTLKDVV WGLNSLFTDL LNFYDPLNIE AAEHHLQDKE DFRDKVDEYI RRYAR Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- 特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- 产品特性
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - 纯度
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- 质量等级
- custom-made
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- 应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- Ube2fb
- 背景
- NEDD8-conjugating enzyme UBE2F-B (EC 2.3.2.34) (NEDD8 carrier protein UBE2F-B) (NEDD8 protein ligase UBE2F-B) (RING-type E3 NEDD8 transferase UBE2F-B) (Ubiquitin-conjugating enzyme E2 F-B),FUNCTION: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. {ECO:0000250}.
- 分子量
- 21.4 kDa
- UniProt
- Q3UWQ3
- AlphaFold
- Q3UWQ3
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