RNF10 Protein (AA 1-804) (His tag)
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- 抗原 See all RNF10 蛋白
- RNF10 (Ring Finger Protein 10 (RNF10))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-804
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This RNF10 protein is labelled with His tag.
- 原理
- Custom-made recombinant Rnf10 Protein expressed in mammalian cells.
- 序列
- MPQSSPSAAA TASDMDKNSG SNSSSASSGS SKGQQPPRSA SAGPAGESKP KSDGKNSNGS KRYNRKREPS YPKNENFSNQ SRRSNSQKSK TFNKMPPQRG GGSSKPFSSS SNGGRRDEVA EAQRAEFSPA QFSGPKKINL NHLLNFTFEP RGQAGHFEGS GHGGWGKRNK WGHKPFNKEL FLQANCQFVV SEDQDYAAHF ADPDTLVNWD FVEQVRICSH EVPSCPICLY PPTAAKITRC GHIFCWACIL HYLSLSEKTW SKCPICYSSV HKKDLKSVVA TESRQYAVGD TITMQLMKRE KGVLVALPKS KWVNVDHPIN LGDEQLSQYS KLLLASKEQV LHRVVLEEKG ALEQQLAEEK HTPESCFIEA AIQEVKIREE ALSGVAGGGG EVTGVVAALE HLVLMAPLAT ESAFQPRKGV LEYLSAFDDE AAQVCSLDPP GPLALPLVEE EEAVSEPEAC EDAEVADDSL GEGTVGPEMS QEEPITKPGF TQLSSSPCYY FYQAEDGQHM FLHPVNVRCL VREYGSLEQS PEKISATVVE IAGYSMSEDV RQRHRYLSHL PLTCEFSICE LALQPPVVSK ETLEMFSDDI EKRKRQRQKK AREERRRERR IEMEENKRQG RYPEVHIPLE NLQQFPAFNS YTCPSDSALG PTSTEGHGAL SLSPLSRSPG SHADFLLTPL SPTASQGSPS FCVGSLEDDS PFLSFAQMLR VGKAKADGWP KTAPKKDDNS LVPPAPVDSD GESDNSDRVP VPSFQNSFSQ AIEAAFMKLD TPATSDPLSE DRGGKKRKRQ KQKLLFSTSV VHTK Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
- 特异性
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- 产品特性
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Key Benefits:
- Made to order protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - 纯度
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- 质量等级
- custom-made
- Top Product
- Discover our top product RNF10 蛋白
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Ring Finger Protein 10 (RNF10) (AA 1-811) protein (His tag)
custom-made RNF10 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ring Finger Protein 10 (RNF10) (AA 1-811) protein (GST tag)RNF10 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Ring Finger Protein 10 (RNF10) (AA 713-811) protein (GST tag)RNF10 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
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- 应用备注
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- 12 months
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- 抗原
- RNF10 (Ring Finger Protein 10 (RNF10))
- 别名
- Rnf10 (RNF10 产品)
- 背景
- E3 ubiquitin-protein ligase RNF10 (EC 2.3.2.27) (RING finger protein 10) (Sid 2705),FUNCTION: E3 ubiquitin-protein ligase that catalyzes monoubiquitination of 40S ribosomal proteins RPS2/us5 and RPS3/us3 in response to ribosome stalling. Part of a ribosome quality control that takes place when ribosomes have stalled during translation initiation (iRQC): RNF10 acts by mediating monoubiquitination of RPS2/us5 and RPS3/us3, promoting their degradation by the proteasome. Also promotes ubiquitination of 40S ribosomal proteins in response to ribosome stalling during translation elongation. The action of RNF10 in iRQC is counteracted by USP10 (By similarity). May also act as a transcriptional factor involved in the regulation of MAG (Myelin-associated glycoprotein) expression. Acts as a regulator of Schwann cell differentiation and myelination (By similarity). {ECO:0000250|UniProtKB:Q5XI59, ECO:0000250|UniProtKB:Q8N5U6}.
- 分子量
- 88.3 kDa
- UniProt
- Q3UIW5
- AlphaFold
- Q3UIW5
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